Effects Of He-Ne Laser And Enhanced Ultraviolet-B Radiation On LaminB Of Wheat Seedlings

With the large number of polluting gases released by humanactivities, gases remaining in the atmosphere for a long time willcatalytic destruction of ozone in the stratosphere. The destruction ofthe ozone layer led to reach the ground of the solar ultravioletradiation enhancement. Studies show that enhanced UV-B radiationon plant, especially for UV sensitive plants in growth anddevelopment, physiological and biochemical metabolism, geneticslevel have important effects. Therefore,revealed that UV-B on cropdamage and mechanism of action, looking for effective protection isparticularly important.Laser has been studied and applied in manyfields. Low dose of laser irradiation can promote plant growth anddevelopment, physiological metabolism. Wheat, which was thesecondary alimentary crop in all the world. Basilic purpose wouldcome to presentation by research how the He-Ne laser enhancedUV-B radiation influences the wheat.LaminB is a bifunctional protein that is also an important component of the spindle matrix, participate in the nucleus formingand spindle assembly. The ‘ML7113’ seedlings of wheat (Triticumaestivum,cv.)were exposed to He-Ne laser irradiation (L) with(5mW·mm-2)power density after enhanced UV-B radiation(10.08KJ·m-2·d-1). Total protein were extracted in each treatmentgroup. The total protein were assayed by SDS-PAGE and identified byWestern Blot. And analyses the influence of enhanced UV-Bradiation-induced the relative content of laminB in wheat seedlingsdamage repair effects by He-Ne laser.In order to investigate theeffects of UV-B radiation on the relationships between laminBarrangements and dynamic variation of laminB in wheat root-tip cellswas studied with indirect immunofluorescence techniques andconfocal laser scanning Microscopy(CLSM),and thus initially revealedmechanism of laminB in wheat seedlings. The results of the study areas follows:1.The detecting by SDS-PAGE electrophoresis and Western-blotprotein identification techniques,results show that: laminB exists inwheat.2.Analysis of the Western-blot results of the detection levels oflaminB in the four treatment groups of wheat by Quantity Onesoftware, the results showed that:compared with the control group(CK), laminB content is lower by the enhanced UV-B radiation treatment group(B); while single laser treatment group (L), thecontent of lamin B was lower than in the control group (CK) butsignificantly higher UV-B treatment group (B); He-Ne laser and UV-Bcompound processing group(BL), the laminB was higher thanenhanced UV-B treatment group (B), lower than laser treatment alonegroup (L); The results shows that a dose of UV-B radiation candamage the lamin B in the wheat. It may have certain role for repairingthe damaged lamin B irradiated by He-Ne laser in the wheat seedlings.3. In the control group (CK), laminB was filamentous formsdistributed around the nucleus of wheat root tip meristematic zonecells in interphase;while in prophase, metaphase, anaphase andpre-telophse laminB was depolymerization form distributed; inpost-telophase,laminB was polymerization in a position to form themembrane.4.In the enhanced UV-B radiation group,LaminB was dots formsdistributed around the nucleus of wheat root tip meristematic zonecells in interphase; while in prophase,metaphase,laminB wasdepolymerization uneven form,anaphase chromosome appearsaberrations, including "chromosome bridge"，"backward chromosome"," micro-nuclear "," Partition-bundle division " and so on.