Study On The Secondary Metabolites Of Rhus Typhina And Their Bioactivity

Rhus typhina fruit extraction parts including: acetic ester part, n-butyl alcohol partand petroleun ether part were studied with method of hypha growth rate, DPPHradical scavenging ability, and brine shrimp death rate to value their antifungalactivity, antioxident activity, and cytotoxicity.Chemical compounds from bioactive extract parts of Rhus typhina fruit wereisolated. The main isolation methods including: Sephadex LH-20, silica gel, andreversed-phase Rp-C18column chromatography together with TLC and HPLC asassistant means. The isolated compounds were identified by means of spectroscopictechniques including IR, UV,1D and2D-NMR (1H-NMR,13C-NMR,1H-1H COSY,HMQC, HMBC and NOESY) as well as mass spectmroetry (HR-ESI-MS ESI-MSand EI-MS,) with great help of database DNP and Antibase. Last but not least,antifungal activity, antioxidant activity, and cytotoxicity of pure compunds werestudied. Results are following behind:①Acetic ester part showed marked bioactivity after three kinds of activity assaies.What’s more, acetic ester part showed certain broad-spectrum antifungal property.After that, n-butyl alcohol part showed good antioxedant and antifnugal activity.Houever, benzinum part exhibited weak bioactivity.②More than28chemical compounds of main bioactive parts were isolated, and20compounds were identified, including（1）Friedelin,（2）1-Friedelen-3-ol,（3）3,5-dihydroxybenzamid,（4）Vanillum,（5）3,4,5-Trihydroxybenzoic acid,（6）3,4,5-Trihydroxybenzoic acid,（7）(2E)-1-(2,4-dihydroxyphenyl)-3-(3,4-dihydroxyphenyl)-2-Propen-1-one,（8）apigenine,（9）quercetin,（10）Methyl3,4-dihydroxybenzoate,（11）O-hydroxybenzoicacid,（12）4-Hydroxybenzaldehyde,（13）7,8-dimethylisoalloxazine,（14）4,4-dimethyl-heptanedioic acid,（15）6,7-dimethoxy-benzopyran-2-on,（16） glaberide,（17）3-[[6-deoxy-3-O-(3,4,5-trihydroxybenzoyl)-β-L-talopyranosyl]oxy]-2-(3,4-dihydroxyphenyl)-5,7-dihydroxy -4H-1-Benzopyran-4-one,（18） Salcolin C,（19）(3β,22E)-ergosta-5,7,22-trien-3-ol-26,27-13C2, and （20） rel-(+)-2-[4-[(1R,2R)-1-[(acetyloxy)methyl]-2-(4-hyxdroy-3-metyhoxphenyl)-2-metheoxythoxy]-3,5-dimoethxyphenyl]-5,7-dihydroxy-4H-1-benzopyran-4-one, that mainly are flavonoids, terpenoids,phenols, and steroids. Compounds （1）,（13）,（16）,（17）,（19）,and（20）were found from Rhus typhina for the first time.③Antifungal activity, antioxidant activity, and cytotoxicity of pure compunds werestudied. Compound（2）to Aspergillus nige,（7）,（13）to Fungi Imperficti,（19）to Fusarium oxysporum,（18）to Penicillium glaucum,（20）to Valsa mali,（12）toSclerotinia sclerotiorum showed specificity antifungal activity, and some compoundsshowed certain broad-spectrum antifungal property.④（17）and（20）exhibit higher DPPH radical scavenging ability then positive controlascorbic acid.⑤Compounds（7）,（9）,（17）, and（20）showed good cytotoxicity close to thatof positive control camptothecin.The present study established the basis for the discovery of plant origin bioactivecompounds and provided theoretical foundation for further utilization of the plantresource.