Studies On Expressions Of WT1and Histone Acetylation In The Development Of Early Embryos And Genito-urinary Organs In Pigs

Wilms’ tumour or nephroblastoma is a paediatric kidney malignancy. The Wilms’tumour1gene (WT1) is the only gene involved in the development of Wilms’ tumourwhich classified as a tumour suppressor gene in Wilms’ tumour. WT1regulates theexpression of several genes implicated in cellular proliferation and development.Additionally, it is essential for renal and gonadal development in mice which invovedin organs development and formation, fanction maintainence and regulation of relateddevelopment genes.A recent study reported that the expression and function of WT1is regulated byhistone acetylation. However, WT1expression levels and the detailed mechanisms ofhistone acetylation controls the expressions of genes during development in porcinegenito-urinary organs are still unknown. Here, we defined the expression pattern,roles of and basic relationship between WT1and global histone acetylation in porcinegenito-urinary cells and organs. The main results were as follows:1. We found that WT1had high expressed in GV and MII embryos, WT1expression was very low in the parthenogenetic blastocyst and we could not detectedWT1expression in the other parthenogenetic embryos. But we found that WT1washighly expressed in the spleen, kidney, ovary and testis of both newborn and adultpigs.2. After treated to porcine kidney fibroblasts (PKF) could enhance expressionlevels of WT1and H4when NaBu concentrations and treatment times were increased.Additionally, WT1and H4expressed at the same place in PKF.3. Obvious changes of organization structure occurred along with thedevelopment of porcine kidneys, ovaries and testes. Additionally, WT1and histoneH4high co-expression in the specific tissues and cells of genito-urinary organs inthe E35d, E60d, newborn and adult pigs, and both WT1and histone H4expressed inthe same areas.Thus, we supposed that the WT1high expressed in embryonic, newborn andadult porcine kidneys, ovaries and testes, which contributed to play a important role in porcine genito-urinary organs development, formation and mature functionmaintainence. Additionally, increasing global histone acetylation levels couldpowerfully improve the expression level of WT1in PKF; hyper-acetylation regulatedthe high expression levels of WT1in different development stages of porcinegenito-urinary organs, which assured genito-urinary organs development, formationand function maintainence in pigs.In this research we determined the expression of WT1and histone acetylation inthe development of early embryos and genito-urinary organs in pigs, and this studywill provide the theoretical and experimental basis to discover the WT1expressionpatter and the regulatory mechanism between WT1and histone acetylation.