| Objective: To explore the therapeutic potential of Curcum in a clinically relevantmouse model of contusion SCI, and to present experimental data for clinical treatmentof acute SCI.Methods:The mouse SCI model was established with modified Allen’s method. Thehealth clean level mouses were divided into three groups: the injury group(acute spinalcord injury group, A group), the treatment group(acute spinal cord injury withmini-dose curcum-treated group, B group) and the control group(simple vertebral plateremoval without spinal cord injury group, C group). The mouses of group B werelavaged by curcum with the dose of200mg/kg after30min and12hours ofinjury,While the mouse of group A and C were treatde with saline lavage as control.Thehindlimb locomotor recovery was evaluated by BMS score system. The spinal cordtissue pathology were observed by HE staining.The expression levels of NF-κBp65wereinvestigated by Western blot.Results:1. BMS score in neurological study: Mouses receiving daily administration ofCurcum after SCI exhibited a significant improvement in hindlimb locomotor abilityas compared to injury group，which showed a decrease than that control group.2.Tissue pathology of HE staining:Mouses receiving daily administration of Curcumafter SCI exhibited less inflammatory cells infiltration in the early time and Lessnerve cells necrosis in the later as compared to injury group，which showed a increasethan that control group.3.Westemblot detection: Mouses with Curcum treatment also showed a significantdecease in NF-κBp65levels when compared to injury group, which indicated a partialincrease observed with control group.Conclusions：ASCI mouses with Curcum treatment resulted in a significant deceasein NF-κBp65levels, infiltration of inflammatory cells, and a significant improvement inhindlimb locomotor ability. The data suggest that Curcum can be considered as a potential therapeutic intervention after SCI. |
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