Evaluation Outside Individual Patients With Breast Cancer Cells Of The Training Of The Induced Nuclear CIK Cells Cbl-b Gene Expression Level And Significance

Objective: Breast cancer is female one of the most common malignancies,although after the operation to chemotherapy and radiation, endocrine therapy for thecomprehensive treatment mainly remarkably enhance the survival local control, butoverall survival is still not satisfied. Primary reason is the recurrence and metastasis.Reports in the literature, immune cells by treatment technology can significantlyenhance the immune function to prevent recurrence and metastasis and prolonging thesurvival time in our country at present is in using the most mature and clinical curativeeffect of the best self is immune cells treatment technology. Among them, the cellfactors inducing killer cells (CIK, cytokine induced killers) treatment of good clinicalcurative effect has been generally accepted, CIK cells the body outside individualnuclear cells after many kinds of cell factors stimulation get a group of heterogeneousgroup of cells. For both T lymphocytes powerful antitumor activity and NK cells theMHC restrictive kill tumor activity etc, and is a new generation of tumor cell biologicaltreatment of choice scheme.Cbl-b (casitas B cell lymphoma-b) is the family members Cbl, T lymphocyte is anegative control signal molecules, foreign weeks T cell regulation plays a key role. AndCbl-b is also adjust T cells activate and tolerance of the key molecules, enhance Cbl-bexpression can induce immune tolerance, prevent autoimmune diseases such as diabetesthe occurrence of multiple sclerosis. Restrain Cbl-b expression or function can changepatients with tumor T cells can state, break their own immune tolerance. Clinicalobservation found that CIK cells to treat process, even if the same training method tolose the same way back to lose cell number also up to standards (1×1010) the sametreatment, but curative effect are different. So we speculate whether the patient’s own because the function of T cells or some factor expression to difference of the trainingfunction appear difference CIK cells, which differ in the curative effect. Thisexperiment in patients with breast cancer through the inspection of the induction ofperipheral blood PBMC CIK cells Cbl-b expression level, to explore different CIK cellsto treat patients, cells Cbl-b gene expression whether have change, and further analyzesthe gene in CIK cells to treat can play an important role.Methods:1. Select patients into groups, fill in informed consent, record the detailnamed disease staging, objective check index, the disease period, has used treatmentsand after, the treatment, patients general situation, The Times of CIK treatment data.2. Such as breast cancer patients peripheral blood extracted a single nuclear cellstotal RNA, extraction of training14days CIK cells of total RNA3. For Real-time polymerase chain reaction (PCR) Cbl-b detection of mRNA levelexpression.4. SPSS13.0statistical analysis.Results:1.In a microscope, PBMC cells in vitro induced by cells appear mothercells, which split phase see more, as time extension cell JiLa increasingly large quantityincreased, tip cell activation proliferation exuberant ability.2.A wish to RanFa count blue cells and show the apoptotic cells rarely seen, livingcells day3cell proliferation about10times, to14days, the cell, amplification of100times.3. Flow cytometry analyzer cellular phenotype analysis, show CD3+CD56+、CD3+CD8+cells ratio increased significantly (p <0.05); CD3+CD4+cellssignificantly (p <0.05).4. Patients CIK cells express Cbl-b mRNA, continuous CIK cells after treatment,the second treatment than the first treatment CIK cells CIK cells express Cbl-bmRNAlevel increased (p <0.001), the third time treatment than the first treatment CIK cellsCIK cells express Cbl-bmRNA level increased (p <0.001), the third time of treatmentwith the second treatment CIK cells of the expression in CIK cells Cbl-bmRNA level nosignificant difference (p>0.05).Conclusion:1. Breast cancer patients peripheral blood PBMC induction trainingCIK cells, CIK cells in vitro proliferation capacity has the very strong expression.2. Breast cancer patients’ CIK cell culture CD3+CD4+after double positive cellspercentage drop, CD3+CD8+double positive cells percentage rise, CD56+CD3+double positive cells percentage rises. 3. All of the patients with breast cancer treatment CIK cells are directly expressCbl-b genes.4. Breast cancer patients with CIK cells to treat the increase of number of, its CIKcells Cbl-b gene expression level also will increase.