Influence Of Low Does Sodium Nitrite On Intestinal Barrier Function Of Rats After Hemorrhagic Shock

Objective (1) By detecting TNF-α and MPO content of the intestinal tissue and calculating the index of quantitative assessment of intestinal mucous membrane injury to observe the effects of low does nitrite on intestinal barrier function of rats after hemorrhagic shock.(2) By detecting NO activity to investigate if sodium nitrite’s potency is correlate with NO.Methods Forty adult male Sprague-Dawley(SD) rats of clean grade weighing200～250g were randomly assigned to four groups, and every group was10rats. Sham operation group(Group S), hemorrhagic shock group(Group HS), nitrite group(Group NI), phentolamine group(Group PI). The rat was heparinized(0.1mg/kg). The carotid artery and carotid vein of rats were exposed and cannulaed. The carotid artery wag cannulated for blood-letting at0.5ml/min to establish the HS model. The MAP was maintained at35to40mmHg for60minutes, collect the blood and conserved in normal temperature. Resuscitate the carotid vein in20minutes, which is carried out by the shed blood and Ringer’s solution to the animals at0.2mL/min for20min, MAP maintained at80%of baseline. A dose of medicines was given when medicine treatment group began to be resuscitate after10min, nitrite(4.8mmol) and phentolamine(0.123mg). Rats in the S group were exposed and cannulaed without resuscitation or shock. After2hours, the content of TNF-α in the intestinal was determined and the pathological changes of intestine were observed. Simultaneously, the levels of MPO and NO in intestine homogenates could be determined.Results Compared with group S, intestinal injury score, expression of TNF-α、MPO and NO increased significantly,(P<0.05). As compared to HS group, the expression of TNF-α, MPO levels were lower in medicine treatment group, but the NO content increase.(P<0.05), injury in group NI is mildest, but NO content is most (P<0.05), between group HS and group PI there is not statistically significant in NO difference (P>0.05).Conclusion (1) Intestinal barrier function of rats can be injuried after hemorrhagic shock, and the shock can induce inflammatory reaction.(2) The issue mentioned above is correlate with TNF-α and polymorphonuclear.(3) After sodium nitrite was given,the increased NO could inbibit the increase in the levels of TNF-α, MPO in intestinal tissue, potential protect the intestinal barrier that suffer from injury.