Effect Of Limb Ischemic Preconditioning On Expression Of VEGF、PDGF-B After Cerebral Ischemia-reperfusion Injury In Rat

Background:cerebral ischemic preconditioning (Ischemia Preconditioning, PC) refers to the brain after a brief sublethal ischemic preconditioning of subsequent severe ischemia of tolerance, which play a protective role. The discovery of this phenomenon for brain protection has opened up a whole new field of research, more important, the study found the sublethal ischemic organ (physical) the lethal ischemia produce to distant organs (heart, brain, etc.) the protective effect of limb ischemic preconditioning (limb, ischemic point backward difference, LIP) LIP can be non-life critical organ ischemic preconditioning protect life critical organs, the mechanism of this protection phenomenon is not yet fully elucidated.Objective:This topic HE staining and immunohisto chemical methods the observed limb ischemic preconditioning (LIP) of the artery ischemia-reperfusion injury (middle cerebral artery occlusion, MCAO) in rats after vascular endothelial growth factor (VEGF), platelet-derived growth factor-B (PDGF-B), to explore the LIP brain protection mechanism.Methods:Healthy male Wistar rats63, weight200g-220g, were randomly divided into three groups:control group, middle cerebral artery ischemia-reperfusion group (the MCAO group:using intraluminal filament MCAO model), limb ischemic preconditioning group (LIP+MCAO group:improved non-invasive blood pressure measuring instrument to give double the femoral artery3cycles of ischemia and reperfusion in preparation before MCAO model, each cycle10minutes-10minutes of ischemia and reperfusion as LIP inducing conditions). Time basis after ischemia and reperfusion, and further divided into the control group, MCAO group and LIP+MCAO group6h,24h,3d three subgroups, each subgroup of nine rats. Rats neurological deficit scores at the corresponding point in time after death. HE staining rat brain tissue pathological changes in brain tissue was detected by Immunohistochemistry method VEGF, PDGF-B, CD31expression.Results:1, compared with the MCAO group, the LIP+MCAO group of rats neurological deficit improved significantly, was statistically significant (P<0.05).2, the light microscope, LIP+MCAO group of histological damage significantly reduced. CD31immune histochemical LIP+MCAO group number of positive cells is higher than the MCAO group was statistically significant (P<0.05), and LIP3days subgroup subgroup than six hours, a24-hour subgroup.4, compared with the MCAO group, LIP+MCAO group more of VEGF, PDGF-B expression was statistically significant (P<0.01), LIP+MCAO group of6hours,24hours,3days subgroup more statistically significant (P<0.01), and24-hour subgroup than6hours, three days subgroups.Conclusion:Limb ischemic preconditioning its brain ischemia-reperfusion injury to produce significant protective effect. Limb ischemic preconditioning marked increase of VEGF, PDGF-B expression, indicating that the protective effect of limb ischemic preconditioning and cerebral infarction, VEGF, PDGF-B expression changes are closely related to.3, the protective effect of limb ischemic preconditioning may be closely related to angiogenesis.