The Preliminary Study On Effectivity And Safety Of Radiofrequency Ablation In Treatment Of Vertebral Tumor

There are two parts in our study.Part1. The variation of Temperature in the spinal canal and vertebral Tumor of Rabbit by radiofrequency ablationObject: To investigate the temperature in the spinal canal and vertebral Tumor of rabbit by radiofrequency ablation.Materials and methods:1. Establishing vertebral tumor models:New Zealand white rabbits were transplanted with VX2carcinoma in the lumbar vertebral body with CT guiding percutaneous puncture technique. Vertebral tumor models were checked after2weeks by CT.2. Experimental grouping and treatment method: The tumor models were randomly divided into2groups. Each group consisted of10rabbits. With CT-guided percutaneous puncture technique, The tumor models in group A was treated3minutes by radiofrequency ablation, the tumor models in group B was treated5minute.3. Observation index:the temperature in the spinal canal and vertebral Tumor of rabbit by radiofrequency ablation was measured and recorded per30second.4. Statistic methods:the temperature in the spinal canal of rabbit at disparate time was analyzed by Repeated Measures Analysis of Variance. The temerature in the vertebral tumor of rabbit at disparate time was analyzed by Repeated Measures Analysis of Variance.Results:1. The temperature in the spinal canal of rabbit at disparate time had statistical difference (P<0.05).2. The temperature in the vertebral tumor of rabbit at disparate time had no statistical difference (P>0.05).Conclusion: The temperature in the vertebral tumor of rabbit reached to the therapeutical and constant one by radiofrequency ablation. The long time of therapy will hurt the nerve in the spinal canal by high temperature.Part2. The preliminary study on effectivity and safety of radiofrequency ablation in treatment of vertebral tumorObject:To investigate the effectivity and safety of radiofrequency ablation in treatment of vertebral tumor Abstract.Materials and methods:1. Establishing vertebral tumor models:New Zealand white rabbits were transplanted with VX2carcinoma in the lumbar vertebral body with CT guiding percutaneous puncture technique. Vertebral tumor models were checked after2weeks by PET-CT. Then the value of SUVmax and SUVmean of each tumor vertebra was measured.2. Experimental grouping and treatment method:The tumor models were randomly divided into2groups. Each group consisted of10rabbits. With CT-guided percutaneous puncture technique, The tumor models in group A was treated3minutes by radiofrequency ablation, the tumor models in group B was treated5minutes, Five rabbits were randomly taken to be checked again by PET-CT at1st day and7th day after operation respectively in each group, and the value of SUVmax and SUVmean of each vertebral tumor was measured again. Then the rabbits were executed and the tissues of tumor were collected for pathological examination.3. Observation index:①The rates of acute paralysis after treatment in2groups were measured and recorded.②The change of the value of SUV: The value of SUVmax and SUVmean of each vertebral tumor in different group and different time was measured and recorded.③HE stain results:The morphological change and necrosis of tumor cells in and near the treatment volume were observed by HE stain.4. Statistic methods:The value of SUVmax and SUVmean of each tumor vertebra before and after RF was analyzed by Repeated Measures Analysis of Variance. The value of SUVmax and SUVmean of each tumor vertebra in different group and different time was analyzed by Analysis of Factorial. Results:1.PET-CT results:The value of the SUV of the tumor vertebra in two groups had statistical difference (P<0.05) among before,1st day and7th day after treatment, the value of the SUV of the tumor vertebra dropped markedly, at the1st day, then it was constant to the7th day, the value of SUV hadn’t statistical difference(P>0.05)in different times after treatment in both of groups. In different group after treatment, the value of the SUV of the tumor vertebra had no statistical difference (P>0.05).2.HE stain results suggested that there was typical necrosis of tumor cells in the treatment volume at1st day after treatment.Far away from the treatment volume we can found partial necrosis of tumor. At7th day after treatment, only single necrotic cells scattered in the tumor.Conclusion: Radiofrequency ablation could significantly lead to necrosis of the vertebral tumor cells in-vivo and induce apoptosis of the tumor cells. Adding therapeutic times will not enhance the therapeutic effect, but will enhance the risk of injury of nerve in the spinal canal.