Research On The Effects Of Full Length Adiponectin On The Proliferation And Apoptosis Of Colon Cancer Cells In The High Glucose Environment

Objective: To observe the effects of the full-length adiponectin (f-APN) with differentconcentrations on the proliferation and apoptosis of colon cancer SW480cells in highglucose environment and discuss the effects of signal passage p38MAPK and activationof cysteinyl aspartate specific proteinase-3(Caspase-3) in the process of f-APN-inducedapoptosis in colon cancer cells apoptosis.Methods: The full-length adiponectin (f-APN) with different concentrations (10μg/ml,20μg/ml,30μg/ml) were used for intervening the colon cancer SW480cell linescultured in the high glucose medium (DEME culture medium with glucose content of4.5g/L) for24h and48h. The experiment was divided into high glucose+0μg/ml f-APNgroup (APN0group, without f-APN), high glucose+10μg/ml f-APN group (APN10group), high glucose+20μg/ml f-APN group (APN20group), high glucose+30ug/mlf-APN group (APN30group), and high glucose+30ug/ml f-APN+p38MAPK retardantSB203580(10μmol/L) group (APN30+SB group).Colon cancer SW480cell linescultured in the low glucose medium (DEME culture medium with glucose content of1.0g/L) was collected as control group (NC group). The cell proliferation activity andapoptosis rate were tested by MTT method and flow cytometer, respectively. And theprotein expression levels of p-p38MAPK and Caspase-3were determined by Westernblot.Result:(1) After24h of f-APN intervention to SW480cells, the cell opticaldensity value (OD value) in APN30group were decreased significantly compared withAPN0group, APN10group, and APN20group（P＜0.05). After48h the OD values inAPN10group, APN20group and PN30group were decreased significantly compared with APN0group（P＜0.01~0.05). And the OD values among APN10group, APN20group and PN30group were significantly gradually reduced （P＜0.05).(2) After24h of f-APN intervention to SW480cells, the cell apoptosis rate ofAPN0group, APN10group, APN20group, and APN30group were3.89%,4.94%,7.43%and8.67%respectively. after48h, the cell apoptosis rate of the above fourgroups were3.96%,6.21%,8.86%and9.47%respectively. The cell apoptosis rate ofAPN30group was significantly higher than the rate of APN0group, APN10group, andAPN20group after24h（P＜0.05）and the cell apoptosis rate of APN10group, APN20,and APN30group were markedly higher than the rate of APN0group after48h（P＜0.05~0.01）, and the cell apoptosis rate among APN10group, APN20group and APN30group were significantly gradually increased（P＜0.05).(3) The results of Western-blot showed that the protein expression levels of activep-p38MAPK and Caspase-3in APN20group and APN30group were much higher(P<0.01) when compared with APN0group, meanwhile compared with APN20group,the protein expression levels of active p-p38MAPK and Caspase-3of APN30groupwere significantly increased (P<0.05), and when compared with APN30group, theprotein expression levels of active p-p38MAPK and Caspase-3of APN30+SB groupwas markedly reduced（P＜0.01~0.05).Conclusions (1) In the high glucose environment, f-APN can restraint the proliferationof colon cancer SW480cells, and can induce the apoptosis. The effect showed thedependent relationship with concentration and time.(2) In the high glucose environment, the promoted apoptotic effects off-APN may be associated with adiponectin by activating p38MAPK pathway, and theactivation of Caspase-3.