Study On The Follow-up Effects Of Cell Cycle And Proliferation Of Mouse Embryonic Fibroblasts Exposed To Power Frequency Magnetic Field

Object:Social modernization and universalization make the electrical equipment an integral part of people’s lives, however, level of power frequency electromagnetic fields, mainly generated by most of the electrical equipment, is consequently great increased in people’s daily life and work place. Public concern related to biological effects of power frequency electromagnetic fields has raised, and a number of research work have being done. Make a correct and objective evaluation of the biological effects induced by power frequency electromagnetic fields is a scientific problem that need to be solved in public health. Epidemiologic investigation and animal experiments and also experiments study of cells show that there may be health-related biological effects of extremely low frequency magnetic fields, however, whether these effects still exist in the long term after ELF MF exposure is still unclear. Cell proliferation is an important process and also an ideal indicator applied in experimental study. By the way, cell cycle distribution alters the cell’s susceptibility to the external stimulus. In the present study, we plan to detect cell proliferation and cell cycle in the long time after power frequency electromagnetic fields treatments, to evaluate the biological effects of power frequency electromagnetic fields, so that to provide reference for research on ELF MF ultimately.Objective:Apply cell proliferation and cell cycle as an indicator to preliminarily evaluate the biological effects of power frequency electromagnetic fields.Methods:MEF cells were cultured for about12hours after reseeding, and then were treated with power frequency electromagnetic fields of50Hz, exposure intensity were0.5mT and2mT, exposure mode included powerline and sine, exposure lasted for8h or24h. Cell proliferation variation and cell cycle distribution would be detected use CCK-8kit and by flow cytometry respectively at0h、12h、24h、36h、48h after power frequency electromagnetic fields treatments. Each test group is equipped with a controlled one, and experiments repeated for three times at least.Results:(1) Cell proliferation results were acquired by CCK-8cell proliferation detection kit:data were analyzed with Covariance analysis by SPSS16.0software, shows that power frequency electromagnetic fields have no impact on cell proliferation under the provided experimental conditions.(2) We further detect cell cycle by flow cytometry:result show that exposure to50Hz,0.5mT power frequency electromagnetic fields of powerline mode for24h got cell cycle of G2/M phase arrest at the following24h after treatment compare to cells in control group; Cells exposed to50Hz,0.5mT power frequency electromagnetic fields for24h after irradiation24h shows a cell cycle block at G0/G1phase; Howerve, we observed cell cycle block at the G0/G1phase after exposure to0.5mT power frequency electromagnetic fields of sine mode for a short time of8h in the long term after exposure at48h.Additionally, either exposure to powerline and sine mode of50Hz,2mT power frequency electromagnetic fields for24h made the percent of cells at S phase increase at the following36h after treatment. Conclusion:We haven’t seen a significant variation both in cell cycle and cell proliferation in the long time after power frequency electromagnetic fields treatments, which may indicate that power frequency electromagnetic fields may not induce significant follow up effects.