Dynamic Changes Of Tgf-b/Smads Signal Transduction Pathway During Hepatocarcinogenesis

Objective:To investigate the significance of TGF-β/Smads signal transduction pathways in the development of diethylnitrosamine (DEN)-induced rat liver cancer.Methods:We intermittently administrated DEN to rats to induce liver cancer, and used light and electron microscopy to observe the morphological changes in the liver during carcinogenesis. We used western blotting and real-time PCR to detect the expression of TGF-p, TβRII, Smad2, Smad3, Smad4, Smad7.Results:Western blotting and real-time PCR results showed that the expression of TGF-β1, TβRII, Smad2, Smad3and Smad4proteins gradually increased, while the expression of Smad7protein gradually declined.Conclusion:TGF-β/Smads1transduction pathways through positive or negative crosstalk and promote and regulate the occurrence and development of DEN-induced rat liver cancer.