Effects Of PAHs On CYP450, AhR, And Immunoglobulin A, G, M In Peripheral Blood

ObjectiveTo study the polycyclic romatic hydrocarbons (PAHs) exposure levels of non-occupation in Tianjin by epidemiology investigation and biological surveillance. To investigate the relationship between the urinary1-hydroxypyrene (1-OHPyr) level and cytochrome P450(CYP450), aryl hydrocarbon receptor (AhR), and immunoglobulin (Ig) A, G, M in peripheral blood of study population. Then to select feasible biomarkers of PAHs exposure of population.MethodsOne hundred people lived in Tianjin without PAHs occupational exposure were recruited in this study. Urine level of1-OHPyr was measured by alkaline hydrolysis combined with high performance liquid chromatography as an internal exposure dose, and cytochrome P450, AhR, and Ig A, IgG, IgM in peripheral blood were measured with ELISA method. Pesonal informations including age, sex, diet, smoking and alcohol drinking, were collected by questionnaire.ResultsIn our study, the urine1-OHPyr level were higher than the exposure limit of ordinary inhabitant (1.15μmol/mol cr), the urine1-OHPyr level in female were higher than in male. The possible reason maybe the difference in lifestyle between male and female.62.5%of women were usually cooking,64.9%of women were passive smokers. The main source of PAHs exposure were lamblack and smoking, and there is interaction between lamblack and smoking.The association between plasma cytochrome P450concentration and urinary1-OHPyr transformed by Log10were weak linear dependence (R=-0.202, P=0.043). There is positive correlation between plasma cytochrome P450and AhR (R=0.546, P=0.001). No correlation was found between urinary1-OHPyr and plasma AhR (P>0.05). The concentration of plasma cytochrome P450and AhR were decreased with PAHs exposure level, but with no statistic difference (.P>0.05).Among the Urine1-OHPyr level in0.025-2.516μmol/mol cr, the concentration of IgA and IgG were increased with Urine1-OHPyr level. While, in the2.517-4.936μmol/mol cr, the concentration of IgA and IgG were decreased (P>0.05). No statistic difference were found in plasma IgM. The study showed that PAHs exposure in small level may increased human humoral immunity compensatorily. High PAHs exposure level may refrain organism immune function. The association between urinary1-OHPyr and plasma IgA concentration and transformed by Log10were weak linear dependence (R=-0.224, P=0.080). The multivariate regression model for plasma IgA concentration showed that urinary1-OHPyr were the main factors inducing IgA concentration change. The independent variable cytochrome P450was not ingress into the regression model. The possible reason is that CYP450and AhR have nothing to do with the immunosuppression of PAHs exposure.ConclusionNon-occupational PAHs exposure effected cytochrome P450and AhR concentration in peripheral blood, cytochrome P450and AhR have nothig to do with the immunosuppression of PAHs exposure. Urinary1-OHPyr concentration used as biological monitoring indicator to evaluate PAHs exposure of coke-oven workers can also to evaluate PAHs exposure of population. Meanahile, it is proposed that cytochrome P450can be used as early warning systerm for identification and prediction of sublethal effects of PAHs exposrue. More samples and more biomarkers are needed to investigate the relationship between PAHs exposure and damage effect.