The Expression And Clinical Significance Of FGFR-2and MMP-9in Bladder Transitional Cell Carcinoma

Objective:To observe the expression of fibroblast growth factor receptor2(FGFR-2) and matrix metalloproteinase9(MMP-9) in transitional cell carcinoma and normal transitional epithelium, and to elucidate the potential relationship between the protein expression of FGFR-2and MMP-9and the clinicpathologic features (age, gender, tumor stage, histological grade and recurrence condition) of bladder transitional cell carcinoma, thus to evaluate the diagnostic and/or prognostic value of FGFR-2as a new tumor molecule marker in bladder transitional cell carcinoma, and to discuss the possible relationship between FGFR-2and MMP-9in epithelial-to-mesenchymal transition of bladder transitional cell carcinoma.Methods:Immunohistochemical staining for FGFR-2and MMP-9was Performed on Paraffin embedded sections from62bladder transitional cell carcinomas and10normal mueosa from Patients who had been treated with surgery in our hospital from2010to2012. In the bladder transitional cell carcinomas,48cases of male, and14cases of female; Aged44to86years old, average65years old; according to the clinical stage,26cases of superficial tumor, and36cases of invasive tumor (T222cases, T38cases, T46cases); according to pathologic stage,52cases of high grade,10cases of low grade;52cases of incipience,10cases of recurrence. The data are analysed by SPSS19.0.Result:1.9cases of normal transitional epithelium are positive in FGFR-2, and the positive rate is90.0%, and30cases of the specimen of bladder cancer are positive in FGFR-2, and the positive rate was48.4%, the two positive rates show statistically difference. In bladder cancer,11cases are in invasive bladder cancer group, and19 cases are in superficial bladder cancer group, thus the difference was statistically significant (P<0.05);22cases are in high Pathologic grade, and8cases are in low pathologic grade, thus the difference was statistically significant (P<0.05);26cases of incipient group, and4cases of recurrence group, and the difference was statistically significant; Age and gender groups had no statistical difference.2. MMP-9is negative in all normal transitional epithelium, and23cases of the specimen of bladder cancer are positive in MMP-9, and the positive rate was37.1%, thus the two positive rates show statistically difference. In bladder cancer,18cases are in invasive bladder cancer group, and5cases are in superficial bladder cancer group, thus the difference was statistically significant (P<0.05);23cases are in high Pathologic grade, and0cases are in low pathologic grade, thus the difference was statistically significant (P<0.05); Incipient group of13cases, and10cases of recurrence group, and the difference had no statistical difference; Age and gender groups had no statistical difference.3. Analysis showed that expression of FGFR-2and MMP-9in bladder cancer has no correlation (r=-0.075).Conclusion:1. The expression of FGFR-2protein in bladder transitional cell carcinoma decreased more obviously than in normal transitional epithelium, and negatively related to the tumor pathological grade and clinical stage. In addition, the protein expression in the incipient group is higher than the recurrent group. From above, we can conclude that FGFR-2is closely related to the malignant degree and the recurrence of bladder cancer, and the molecule could become a new tumor suppressor factors of bladder transitional cell carcinoma. The detection of the abnormal expression of FGFR-2in bladder cancer may be helpful in the diagnosis of bladder cancer, as well as judgement for the prognosis. 2. Comparing with normal transitional epithelium, the expression of MMP-9protein in bladder transitional cell carcinoma is higher, and is closely related to the tumor pathological grade and clinical stage:the higher pathological grade and the clinical stage, and the higher the expression of MMP-9protein. Detection of bladder transitional cell carcinoma tissue of MMP-9protein expression is helpful to the diagnosis of bladder cancer, also helpful to judgment of prognosis of bladder cancer.3. Statistics analysis showed that the expression of FGFR-2and MMP-9in bladder transitional cell carcinoma is not relevant, and this may indicate that, although both the molecules participate in epithelial-mesenchymal transition of bladder transitional cell carcinoma, the steps and stages involved are different, so no direct link occurs.