| Objective1.To analyze the influence of season on level of PAHs and the relative proportion of respiration and diet exposure to PAHs, and assess the health risk of two path exposure to PAHs, ensure relative risk level of two path, to take measures protect human health.2.To study on new biomarker for PAHs exposure and investigate the feasibility of using8-OHdG to reflect the early genetic damage in general population.MethodsGC-MS was used to analyze the concentration of PAHs in particulate matter and food samples as well as concentration of OH-PAHs in urine, ELISA was used to analyze the concentration of8-OHdG in urine. Two independent sample’s Mann-Whitney U test was used to analyze the influence of season on PAHs in particulate matter and food samples, Monte Carlo simulation was used to assess the cancer risk of respiration and diet PAHs exposure. Spearman function was used to analyze the correlation between OH-PAHs and8-OHdG, Euclidean distance was used to analyze the correlation between OH-PAHs and corresponding PAHs in particulate matter and food samples, multiple linear regression was used to analyze the influence of PAHs on8-OHdG in urine.Results1.The influence of season on PAHs in particulate matter has statistical significance(P<0.05), the concentration of low ring PAHs in summer was higher than in winter, the high ring PAHs was higher in winter than in summer. The influence of season on Acenaphthylene, Fluorene,3,4-Benzofluoranthene, Anthracene, Chrysene and11,12-Benzofluoranthene in food has statistical significance(P<0.05), and was higher in summer than in winter besides Acenaphthylene. Respiration path account2percent and diet account98percent in summer, Respiration path account5percent and diet account95percent in winter.2.The concentration of OH-PAHs in urine decreased in the following order:1-hydroxynaphthalene>2-hydroxynaphthalene>9-hydroxyfluorene>2-hydroxyfl uorene>3-hydroxyphenanthrene>2-hydroxyphenanthrene>3-hydroxyfluorene>4-hydr oxyphenanthrene>1-hydroxypyrene>b,c-dihydrodiolsphenanthrene>6-hydroxychryse ne, the OH-PAHs of low ring Naphthalene, Fluorene and Phenanthrene were the main component in urine.1-hydroxypyrene and other OH-PAHs was significant correlated, Spearman’s rank correlation coefficient was between0.454and0.902(P<0.01). The correlation between OH-PAHs and corresponding PAHs in particulate matter decreased in the following order:l-hydroxypyrene>6-hydroxychrysene>2-hydroxynaphthalene>2-hydroxyfluorene>b,c-dihydrodiolsphenanthrene. The correlation between OH-PAHs and corresponding PAHs in food decreased in the following order:1-hydroxypyrene>total hydroxynaphthalene>6-hydroxychrysene> b,c-dihydrodiolsphenanthrene>2-hydroxyfluorene. The correlation between other nine kinds of OH-PAHs and8-hydroxy-2’-deoxyguanosine has statistical significance(P<0.05) besides1-hydroxynaphthalene and b,c-dihydrodiolsphenanthrene.3. The influence of PAHs,1-hydroxynaphthalene, b,c-dihydrodiolsphenanthrene, age, gender, smoking, drinking and exercises on8-hydroxy-2’-deoxyguanosine has no statistical significance, Body mass index and other nine kinds of OH-PAHs has statistical significance(P<0.05), As the decrease of body mass index and the increase of OH-PAHs the level of8-hydroxy-2’-deoxyguanosine increased. Multiple linear regression analyze did not find the influence of OH-PAHs and body mass index on8-hydroxy-2’-deoxyguanosine.4.Incremental lifetime cancer risk of respiration PAHs exposure for summer and winter was between-2.37×10-8and2.53×10-6as well as between-1.81×10-6and1.78×10-5respectively. The mean for Incremental lifetime cancer risk of respiration PAHs exposure in summer and winter was1.70×10-7and2.16×10-6respectively. Incremental lifetime cancer risk of diet PAHs exposure for summer and winter was between2.27×10-8and7.48×10-5as well as between4.87×10-11and1.42×10-5respectively. The mean for Incremental lifetime cancer risk of diet PAHs exposure in summer and winter was2.60×10-6and1.34×10-6respectively.Conclusion1.Incremental lifetime cancer risk of diet PAHs exposure was higher than respiration PAHs exposure in summer, incremental lifetime cancer risk of respiration PAHs exposure was higher than diet PAHs exposure in winter, but the risk level was still acceptable.2.Simultaneous determination of1-hydroxypyrene,2-hydroxynaphthalene,2-hydroxyfluorene and b,c-dihydrodiolsphenanthrene can be more accurate and comprehensive in relecting respiration and diet PAHs internal exposure level.8-hydroxy-2’-deoxyguanosine can used as sensitive biomarker to reflect early genetic damage caused by PAHs in general population. |
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