Microencapsulation Of IL-1Ra Gene Transfected Bone Marrow Derived Mesenchymal Stem Cells

ObjectiveThe study of sodium alginate material wrapped turn IL-1Ra gene from rat bone marrow mesenchymal stem cells microencapsulated whether the parcel of non-autologous cells to avoid immune rejection, the secretion of IL-1receptor antagonist inhibits inflammation..Methods1. By their plasmid PLXRN-1L-1Ra and transfected the PT67cell strain and virus extraction.2. From rat bone marrow (BMSCs) were isolated and cultured mesenchymal stem cells, the use of high-voltage electrostatic method of production of encapsulated cells alginate-polylysine-alginate (APA) microcapsules, fluorescence microscopy cells were observed in the microcapsules and4weeks of survival.3. Microcapsulated Biological Effects of mesenchymal stem cells in the bone marrow; ELISA determination of the recombinant product secreted into the extracapsular IL-1Ra content.4. Microcapsulated IL-1Ra immune isolation of mesenchymal stem cells in the bone marrow; APA microcapsule membrane permeability of the determination.Results(一) It was found that the plasmid was transfected into the power is between70%-80%, pt67after transfection of cell lines growing well, no cell death was observed under a fluorescence microscope.(二) Sodium alginate concentration of1.5%in a cell concentration of3x106/ml, centrifuged for2, high voltage electrostatic method Country of MSCs cells microcapsules can be formed in good shape, the appropriate microcapsule size. (三) Microcapsules parcel28days after stem cell, cell survival still visible under a fluorescence microscope. The ELISA detected parcels until28days after the cumulative secretion of IL-1Ra rising.(四) Transparent wall complete APA microcapsules not marked with fluorescent yellow IgG antibodies was found.Conclusion:IL-Ra gene in bone marrow mesenchymal stem cells transfected with the sodium alginate and other material wrapped to make the microcapsules long-term secretion of IL-1receptor antagonist, and have a good immune isolation effect is expected to be suppressed or treatment of rheumatoidarthritis new gene delivery technology platform.