Genistein’s Effect On Intracerebral GLT-1after Intracerebral Hemorrhage In Rats

Objective:To observe the genistein’s effect on Glutamate concentration and GLT-1in cerebral hemorrhage models of rats in order to evaluate the protective effect of genistein on the brain and its possible mechanism.Methods:72male Sprague-Dawley rats (body weight0.25-0.30Kg) were randomlydivided into sham operation group, model group, Gen group, DMSO group.Sham-operated group by surgery was injected intracranially with saline as a control,model group, Gen group, DMSO group type IV with collagenase to produce model ofintracerebral hemorrhage. Gen group after intraperitoneal injection of genisteinsolution (50ug/g solution,diluted with DMSO), DMSO group was injected withDMSO solution. On the first postoperative day, fourth day and seventh day,the rats’behavior neurological scores were observed and sampling measurement indicatorswere recorded:1. In the light microscope by HE staining intracerebral hemorrhage inrats brain tissue pathology damage and changes in astrocytes;2.Immunohistochemistry tag Glutamate transporter GLT-1in the light microscopeobservation of GLT-1after cerebral hemorrhage, and GLT-1protein was detected byWestern Blot content.3.cerebral hemorrhage surrounding tissue Glutamateconcentration detected in each time point, the concentration of lactate dehydrogenase.Results:The study found that: SD rat brain hemorrhage, brain tissue between Glutamateconcentrations and neurologic deficit score was positive linear correlation; Gen treatment compared with the model group concentration of Glutamate concentration in the brain tissue of a decline, in the7th Timeless Glutamate concentrations decline more pronounced, with the model group, the difference was statistically significant (P <0.05).Gen with model group, LDH activity reduction (P <0.05). Immunohistochemical results and Western Blot display model group, DMSO group GLT-1expression were down compared with the sham group, but the the Gen group and the model group comparedto GLT-1upregulation (P <0.05). At the same time point Glutamate, LDH, GLT-1respectively between Gen group and DMSO group have no significant varience(p>0.05).Conclusion:1.After intracerebral hemorrhage in rats the GLT-1is downregulated.2.Intervention of genistein leads to GLT-1upregulation and decreasedGlu concentration in brain after cerebral hemorrhage in rats.