Research About Inhibition Of Gastric Cancer Cell Line BGC-823Proliferation By Diosbulbin B

AIM:By detecting Diosbulbin B to the influence of huan gastric cancer BGC-823cellproliferation in vitro culture.detection of cell death observe apoptosis morphologicalchanges.Explore Diosbulbin B on human gastric cancer cells in vitro inhibitoryeffect. Tofurther clarrify the mechanism of Diosbulbin B resistance of gastric cancer.In order tobetter develop a new generation of gastric cancer drugs.METHODS:Methods Human gastric cancer cell line BGC-823were treated with variousconcentrations of Diosbulbin B for24h，then cell proliferation was detected using MTTas-say．Then determine the antioxidant antivity of Diosbulbin B;Flow cytometry cellsinduced apotosis;Cell morphology observed underted microscope.RESULTS:Results The cell inhibitory rate was12.48%，30.1%，43.02%，51.41%after treated byDiosbulbin B at10，20，40，80μmol/L concen-tration levels for24h，IC50valuewas(70.075±1.846)μmol/L．The concentration of Diosbulbin B correlated well with itsactivity of scavenging DPPH free radical(r2=0．964)．Diosbulbin B As well as b (BGC-823gastric cancer cell apoptosis can be induced in20or40g/mL mu early and late apoptosis canbe induced under Observed under inverted biological microscope (10,40) treatment groupcell morphological changes after24h of cell shrinkage, it become visible and piece surfacefloating in solutionConclusionDiosbulbinB had a dose-dependent inhibitory effectwas obvious,on the proliferation ofhuman gastric cancerBGC-823cells in vitro; The anti-tumormechanism may beasantioxidantand cell apoptosis.