| 【Objective】1.To investigate exogenous estrogen （17β-estradiol） for Mfn2expression inbreastcancer cells.2.To find the therapeutic significance of the Mfn2gene in breast cancer,especially inER receptor negative breast cancer cells,and to explore the upstream regulatorymachnisms,to look for the potential targeted therapy sites.【Metholds】1. In vitro cultured cells,with different concentrations of estrogen levels stimulatebreast cancer cells.2.At a certain point in time, the MMT method for detect the rateofcellproliferation,western blot for detect Mfn2protein expression in the cells in each group, Real time-PCRfor detect cell Mfn2expression at the mRNAlevel.3.Mapping with the GraphPad prism5software and SPSS17.0statistical analysisfor the data.【Results】1.In the proliferation rate,after various concentrations of estrogen stimulated MCF-7cells,the proliferation rate of each group has an increase,the increase of1×10-8mol/L themost significant;whereas in MDA-MB-231cells,each group have an impact on the rate ofproliferation, but the difference was not statistically significant,with the respect to thegroup,the proliferation rate is decreased in the concentration of1×10-6mol/L、1×10-7mol/L.It is worth noting that the decline respect to the control group is statistically significant at aconcentration of1×10-6mol/L after48h。 2.After the estrogen stimulated cells,in the amount of mRNAand protein levels ofMfn2have risen,and was positively correlated with the time.【conclusion】In the ER receptor-positive MCF-7cells，the17beta-estradiol can stimulated cellproliferation,but the proliferation rate was not increased with increasing hormonelevels;while,in the ER receptor negative cells had no significant effect,but decreased in the1×10-6mol/L, Presumably had relationship with some proliferation suppressor gene. |
PDF Full Text Request