| Objectives: Multiple sclerosis is a common central nervous system autoimmunediseases. Because the incidence and pathophysiological changes of the Rats withExperimental Autoimmune Encephalomyelitis are the same as Multiple sclerosis, Soclinical recognized using EAE model as the experimental animal model of MS.Inorder to further explore the Zi yin Gu ben Decoction on acute spinal cord injury inrats EAE protection mechanism, so as to provide experimental evidence for clinicalapplication of Zi yin Guben Decoction. We through to observe the effect of Zi yinGuben Decoction on acute EAE after spinal cord injury in rats and the expression ofNogo-A protein and combination the score of neural function damage,and to observe thepathological changes of spinal cord injury after HE staining.Methods: The60female wistar rats were randomly divided into6groups byrandom digits table：The blank group、model group、 hormone group、 traditionalchinese medicine high dose group、 middle dose group、 low dose group、10ratsin each group. We establish the EAE model by use the immune antigen Inoculationinto the female waster rat dermal insole lower limbs which is Composed of thefresh guinea pig spinal cord homogenate plus complete Freund’s adjuvant, At thesame time injection of pertussis solution increased blood-brain barrier permeability,which makes the model more high success rate. We will observe the rats weight,food intake and behavioral changes and the corresponding score when the modelafter preparation. To observe the pathological changes by HE staining of spinal cordand to detect the expression of Nogo-A by immunohistochemistry (SP) method.Results：1.Changes of neurological behavior The rats in the blank group without obvious behavioral change. EAE of model group rats neurological behavior performance forthe reduce food intake, weight loss, incontinence, paralysis of limbs and othersignificant pathology in7days after the injection of antigen;Compared with themodel group，Traditional chinese medicine high dose，medium dose group and thehormone group were showed significant neurological behavior in ninth days，On the14day reached a peak in neurobehavioral changes,There is a significant difference inmodel group and treatment group (p<0.05)；Treatment group, neurological behaviorscore lower than those in the model group;No significant difference between thehormone group and high dose group and middle dose and low dose group(p>0.05);Nosignificant difference in middle dose and high dose group (p>0.05),There is asignificant difference in hormone group and middle dose and high dose group(p<0.05).2.Results pathological changes Spinal cord tissue of rats in blank was noinflammatory cell infiltration, demyelination changes;Spinal cord tissue of rats inmodel group showed obvious inflammatory cells change in cuffing infiltratio,neuralcell vacuolar degeneration and neuronal shrinkage, and there are demyelination andproliferation of glial cells surrounding blood vessels，at the same mine,we can see theAxonal swelling、degeneration.Compared with the model group,pathological changes intreatment group was improved.We observed that the number of inflammatory cellsdecreased significantly, no obvious cell degeneration, demyelination lesionssignificantly reduced, spinal cord tissue cells arranged relatively neat.3.The expression of Nogo-A protein The Nogo-A protein is axonal regenerationinhibitory factor in neurons, oligodendrocytes cytoplasm. The positive expression wasbrown,With oligodendrocyte proliferation and release large amounts in self repairingnerve system of the body. The model group showed Nogo-A protein expression wasstrongly positive,in contrast,The expression of Nogo-A protein relatively reduced inthe hormone group and high, low,medium dose of Chinese medicine.No significantdifference between the hormone group and high dose group in the treatment of eachgroup (p>0.05);There is a significant difference in middle dose and high dose group(p<0.05);There is a significant difference in middle dose and low dose group (p<0.05).Conclusion:1.This experiment successfully established the animal model of multiple sclerosis;2.Ziyin Guben Decoction preventive treatment of the acute phase of EAE rats canreduce the incidence and make the incidence of time delay; 3.Ziyin Guben Decoction can reduce the inflammatory changes of spinal cord tissue ofrats with EAE and make he body weight of EAE rats under control，throughinhibition of neurite growth inhibitory factor (Nogo-A) in the acute stage to make theNervous system repaired;4.No significant difference between the inhibitory effect on Nogo-A expression in thehigh dose of Ziyin Guben decoction and hormone,but high dose compared withmiddle dose have obvious difference, so there is a certain correlation between clinicalon multiple sclerosis treated with traditional Chinese medicine and dose. |
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