Design Synthesis And Preliminary Characterization Of Hydrolytic Enzyme New Chromogenic Substrate

Aim at ELISA (Enzyme-Linked-Immuno-Sorbent Assay;ELISA)analysis of the efficiency is low;the measurable range is narrowshortcomings. Put forward the use of isoabsorbance wavelength of the newmethod of simultaneous determination of multi-component;Single-ChannelMultiple-Component Simultaneous ELISA;SCMCS-ELISA. Screened outaccording to the commonly used immunoassay enzyme alkalinephosphatase;serum aromatic esterase;alpha-D-galactosidase enzyme;andbeta-D-galactosidase as a candidate hydrolase. To select commonly usedhydrolase substrate p-nitrophenyl acetate;p-nitrophenyl phosphatedisodium salt;p-nitrophenyl alpha-D-galactosidase as a candidate substratecomposition.According to the candidate hydrolase corresponding substrate and theproduct p-nitrophenol isoabsorbance wavelength design expectedcombination with hydrolase substrate. Determination of change of theabsorption peak of the new substrate;substrate does not comply with therequirements of the isoabsorbance wavelength in the nucleus to bemodified functional groups;and ultimately comply with requirementsisoabsorbance wavelength wavelength substrate4-nitro-1-naphthyl phosphate and4-nitro-1-naphthyl-β-D-galactopyranoside and the rest isexpected to further modification and application of the new substrate.Determination of two new substrates michaelis constant.the buffer effect ofpH effect and kinetic properties infer new substrates for hydrolyticenzymes can be used as the the SCMCS-ELISA.