Primary Survey Of VE-cadherin Function In Mefastasis And Invasion Of Non-small Cell Lung Cancer

Bakground：To investigate the mechanism of the metastasis andinvasion on lung cancer and to find new target are important to contributatedto make out clinical therapy strategy. VE-cadherin is anendothelium-specific cell-cell junctional protein that plays a critical role invascular barrier function and angiogenesis.Our preliminary study found thatVE-cadherin has a close relationship with the angiogenesis of non-small celllung cancer （NSCLC）.We infer from that VE-cadherin play an importantrole on the invasion and metastasis of NSCLC.Morover，despite someresearch of VE-c adherin， we knew litterabout of whether VE-cadherincontributes to the metastasis and invasion on NSCLC.Objective: To detect the expression of VE-cadherin in differentnon-small cell lung cancer cell line with distinct invasion performance and toinvestigate the effect of the VE-cadherin silence on the invasion andmigration of PG cell（non-s mall cell lung cancer cell line）.Methods：1.The expression of the VE-cadherin mRNA and protein was detected by quantitative PCR and western blot in three non-small cell lung cancer cellline which are PG （highly invasive）, H1299（normally invasiveability）a nd PA（lowly invasive）(PG vs. H1299and PA).2.Then we explored the effects of the expression of VE-cadherin on theinvasion and migration of PG cell line by RNA interference.Results：1.The expression of VE-cadherin in PG cell line which had highlyinvasive capacity was the highest than that of H1299cell line which hadnormally invasive capacity. and the PA cell line which was lowerinvasivly.The difference between them was statistically significant(P<0.001).2.The result of Transwellchanber matrigal invasion assays revealed thatthe invasive cells of control group and treatment group we re34．33±1．764and86．00±4.583respectively. The difference between the twogroups was significantly (P<0.001).3.The consequence of the Scratch damage experiment showed that themigration abilities of control group and treatment group had no obviousdiffere nce in the first24hours(P=0.8246>0．05). But after48hours or72hours, the migration distance of control group was farther than the one oftreatment group. The difference was statistically significant (P<0.000).Theinvasion and migration of PG cell were down regulated by the reduction ofthe expression of VE-cadherin. Conclusions:1. RNA interference can be effectively silenced the expression ofVE-cadherin in NSCLC cell lines.2.The expression levels of VE-cadherin in NSCLC cell lines are closelyrelate d with their potential metastasis capacity， the higher invasion，themore expression.3.VE-cadherin plays a critical role in the invasion and migration of lungcancer cell.4.To silence the target gene of cancer cell by RNA interference ishopefully to become a new cancer. treatment method.