Effect Of Heparin To The Banna Minipig Intracerebral Hemorrhage Animal Model

Object:Autologous blood injection method is adopted to establish theBanna minipig intracerebral hemorrhage animal model,to explore the effectof heparin to animal model on neuroethology,imaging and histopatholog,thatassess the stability and rationality of animal model.Methods:Select30malebanna minipigs, grouped by random number table method, the animal can bedivided into three large groups and its corresponding to two time points(day1and day7) group, a total of6groups.Anticoagulant group (n=10) andno anticoagulant group (n=10) using two times injection method to injectanticoagulation/no anticoagulant blood into the left basal ganglia, makinganimal model of intracerebral hemorrhage and were in control group (n=10)for comparison.All the animals perform neurobehavioral score, magneticresonance imaging on postoperative day.1. Animals in day1group wouldbe put to death and then perform pathologic examination. Correspondingly,Animals in Day7group would be put to death after finish the above checksagain. Results:1. We create20Banna minipig intracerebral hemorrhageanimal model Successfully.2.There is no statistical difference between allweights of the Banna minipigs.3.On postoperative day1,the Purdyneurological deficit scores of animals in Anticoagulation group,no anticoagulant group are greater than the control group(P<0.05).and thescores of anticoagulant group are greater than no anticoagulant group;Onpostoperative day7,there is no statistical difference between all groups.4.The change of hematoma volume: Hematoma volume and edema volumewas calculate on MRI T2flair:On postoperative day1, hematoma volume ofno anticoagulant group are greater than anticoagulant group P<0.05,inanticoagulant group,the hematoma volume of day7is greater than day1P<0.05).However, there is no significant change in the no-anticoagulantgroup P>0.05.5.The change of edema volume:On postoperative day1,edema volume of no-anticoagulant group are greater than anticoagulantgroup P<0.05,in no-anticoagulant group,the edema volume of day7isgreater than day1P<0.05),However, there is no significant change in theanticoagulant group P>0.05).6.On postoperative day1, the edema volumeand hematoma volume were put in Linear regression analysis,we find theyare not related.7.Compare the hematoma volume measured in two ways(histology and MRI),there is no statistical difference between them.8. Thequantity of neuron which is positive in Nissl’s staining:On postoperative day1,the neuron number of anticoagulant group and no-anticoagulant group isless than the control group P<0.05).8.the results of MRI:On postoperativeday1,there is a circular-like T2*WI,T2Flair Low signal area in left basalganglia,where a band arounds,which is T2*WI T2Flair high signal.Onpostoperative day7,circular-like area become T1WI high signal,and T2*WI low signal.At the same time,the band becomes moer wider.10.The resunlt ofpathological examination:specimen in general view,we can see a hematomain left basal ganglia of every ICH animal model.perihematoma tissu isdisorder,Perivascular inflammatory cell accumulation,Serious edema, theNeurons’ normal structure disappearing, astrocyte and minute vesselproliferation during HE staining.Nissl substance reduce,Neuronsedema,nucleus disappearing during Nissl’s staining.Conclusion:a smallquantity,more times, quick no-anticoagulant blood injection method used tomake ICH animal model is simple,which is characterized with high successrate,repeatability,stability.2. Heparin can reduce perihematoma edema,butlead to progressive enlargement of hematoma.3.MRI measured hematomavolume is consistent with actual hematoma volume.Therefore, we can useMRI to observe ithe dynamicchanges changes of the hematoma.