| Objective:Atopic dermatitis and eczema are common diseases in dermatology, which occur at any age and tend to last for several years, attacking repeated. Domestic and international epidemiological investigation reports indicate that the incidence rate keep rising, and it become to a global health problem. The patients had varying degrees of itching and skin lesions. The etiology and pathogenesis is not clear yet, and lack of effective treatment. It perplex physiological, psychological and daily life of patient, affecting the quality of life seriously. The pathogenesis research of AD and eczema focused on the imbalance of Thl/Th2cell subsets, but not much on the relationships between Thl, Th2and thymic stromal lymphopoietin(TSLP). And also our knowledge is limited in TSLP’s effect on the balance of Thl/Th2cell subsets in such diseases. To investigate the role of human TSLP in the pathogensis of AD and eczema, and discuss the relationships between human TSLP and Thl as well as Th2cells.Methods:Using case-control study, we collected peripheral blood samples3ml from patients with AD and eczema and healthy person. And let them stand for blood clotting in natural condition, then dealt them with low speed centrifugation(1000r/min,15-20min), extracted serum and stored them in-80℃ultra-low temperature freezer to be detected. ELISA technique was used to measure the levels of TSLP, IL-4, IFN-y in the sera of every group. The difference in the comparison between each groups, and correlation analysis was used to investigate their association between them. Data were analyzed by SPSS17.0software. The t-test was used for the comparison between the measurement data, and Pearson correlation analysis was used for the associativity of each sample.Results:1.Blood serum levels of the immune factors:(DTSLP:atopic dermatitis group:184.61±35.90pg/ml, eczema group:165.03±23.49pg/ml, normal control group:136.49±18.59pg/ml. The blood serum levels of TSLP in group of atopic dermatitis was significantly higher than normal control group, the difference was statistically significant(P<0.05); The blood serum levels of TSLP in group of eczema was significantly higher than normal control group, the difference was statistically significant(P<0.05); The blood serum levels of TSLP in group of atopic dermatitis was higher than eczema group, the difference was statistically significant(P<0.05).②IL-4:atopic dermatitis group:81.46±9.26pg/ml, eczema group:76.09±6.73pg/ml, normal control group:35.11±7.86pg/ml. The blood serum levels of IL-4in group of atopic dermatitis was significantly higher than normal control group, the difference was statistically significant(P<0.05); The blood serum levels of IL-4in group of eczema was significantly higher than normal control group, the difference was statistically significant(P<0.05); The blood serum levels of IL-4in group of atopic dermatitis was higher than eczema group, the difference was statistically significant(P<0.05).③IFN-y:atopic dermatitis group:46.88±10.92 pg/ml, eczema group:45.00±9.50pg/ml, normal control group:26.61±11.65pg/ml. The blood serum levels of IFN-y in group of atopic dermatitis was significantly higher than normal control group, the difference was statistically significant(P<0.05); The blood serum levels of IFN-y in group of eczema was significantly higher than normal control group, the difference was statistically significant(P<0.05); The blood serum levels of IFN-y in group of atopic dermatitis was higher than eczema group, the difference was no statistically significant(P>0.05).2. Correlation analysis between TSLP and IL-4, IFN-y in serum:①In the atopic dermatitis group:There was a positive linear correlation between serum level of TSLP and IL-4(r=0.519, p<0.05); There was a negative linear correlation between serum level of TSLP and IFN-y (r=-0.726, p<0.05).②In the eczema group:There was a positive linear correlation between serum level of TSLP and IL-4(r=0.627, p<0.05); There was a negative linear correlation between serum level of TSLP and IFN-y (r=-0.785, p<0.05).Conclusion:Using case-control study, we compared to blood serum levels of TSLP, IL-4, IFN-y in patients AD and eczema and healthy person. Preliminary findings:①Three cytokines in atopic dermatitis group and eczema group serum are all significantly higher than normal control group, prompting Thl/Th2cell subsets imbalance does exist in these two diseases.②SLP could promote EL-4expression and inhibit IFN-y expression, prompting TSLP might play a role in the pathogenesis of AD and eczema.③he levels of IL-4and IFN-y in serum were higher in Case group than normal control group, prompting these cytokines might take an important role in the pathogenesis of AD and eczema. |
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