Analysis Of Constituent Ratio Of The Free Bile Acids In Patients With Gallstone Disease

Background: Gallstone disease is one of the most common clinicaldigestive diseases. It is formed due to liver metabolic disorders or biliarymotor function disorders, resulting in the solid component, which hasoriginally dissolved in bile precipitates. The results of the diseases alsoinclude abnormal mucin secretion of biliary epithelial cells, lipidmetabolism disorders, bacterial and parasitic infections, promote/proteinsuppression system imbalance and abnormal gene regulation.Throughseveral generations of researchers to explore all aspects of gallstone disease,so far have made some progress in the formation mechanism of the gallstonedisease on the aspects of cholesterol metabolism disorders, gallbladderkinetic disorders, abnormal secretion of mucin gene regulation disorders,hereditary. However, specific mechanism of gallstones is still not very clear.Most of the studies were analyzed for bile acid in the human body a varietyof biological specimens (including serum, urine, bile, faeces). Almost allpatients suffer from abnormal bile acid metabolism. The stone recorded avariety of information in the process of formation. We can gain accurateunderstanding of the dynamic changes of the nuclear processes and providea theoretical basis for the different mechanisms of different types of stoneformation from lithogenic bile stones in the bile acid component byanalyzing free bile acid component from stone samples.Objective: Study different types of stones of patients and difference ofconstituent ratio of the free bile acids in bile and gallstone samples of thesame patient. Explore the relationship between the formation of the free bileacid constituent ratio changes with different types of gallstones combinedwith clinical and biochemical indicators. Method: Collect gallstones samples from63different parts. Classifythe stones by Fu Peibin Act optical microscope into Cholesterol stones andbile pigment stones. Measure the component of free bile acid in the twogroups of bile and stone samples and constituent ratio of the free bile acidsin bile and gallstone samples of the same patient by high performance liquidchromatography mass spectrometry.Result:1. The average concentration of FBA in bile samples of cholesterolgallstone group is less than that in bile pigment stones group. The result ofUDCA is the opposite. The formation of FBA in bile samples of cholesterolgallstone group is: CDCA>UDCA>CA>DCA>LCA. While in bile pigmentstones group is: CDCA>CA>DCA>LCA>UDCA2. UDCA in stone samples of cholesterol gallstone group is higher thanin bile pigment stones group. The result of CA(%), CDCA(%), CA/CDCA isthe opposite. FBA and LCA in the2groups have no statistically significant.The formation of FBA in bile samples of cholesterol gallstone group is:CDCA>LCA>UDCA>CA>DCA. While in bile pigment stones group is:CDCA>CA>DCA>LCA>UDCA3. The concentration of CA in bile sample is higher than in the stonesample of the same patient. So do CA/CDCA and DCA. The result of UDCAis opposite.4. The concentration of UDCA in stone sample is higher than in thebile sample of the same patient. CA(%), CDCA(%), CA/CDCA has the sameresult, DCA(%),LCA(%)is the opposite.Conclusion:1. Analysis of gallstones in the bile acid composition is simple, whichtruly reflects the dynamic changes of bile acid component in the process ofgallstones into the stone.2. In the process of stone forming, the constituent ratio of FBAchanges.3. The changing of constituent ratio of FBA, especially the reducing ofUDCA proportion of hydrophilic bile acid relates to the forming of bilepigment stones needs further validate. 4. Bile acid component ratio determination to play a supporting role tospeculate types of gallstones.