| The ureteral obstruction is common disease in urinary surgery, for the kidney damage caused by ureteral obstruction, the priority is to relieve the ureteral obstruction, and one of the most critical consideration is whether ureter recanalization could be realized after removing the obstruction, in other word, whether the damage of ureter can be repaired appropriately. Mesenchymal stem cells (MSCs) is a kind of adult stem cells, has been currently the attention of used in the treatment of various diseases research and obtained a wide range of attention, source of MSCs diverse, one of the most common source is from the bone marrow, the bone marrow mesenchymal stem cells (BMSCs).Object:To study the methodological effect of BMSCs for ureteral injuries repair.Methods:Firstly, use micro vascular clamp to set up BALB/c mice left ureteral obstruction model, take out the microscopic vascular clamp to lift left ureteral obstruction after10days, then these mice were randomly divided into experimental group and control group. Green gene of BALB/c mice BMSCs transfected by dual reporter fluorescent protein, green fluorescent protein (GFP) with luciferase (Luc) were transplantated immediately through the renal artery after removing microscopic vascular clamp from the experimental group; control group mice were after microscopic vascular clamp was removed immediately to close the incision, without transplanting BMSCs. Then using the in vivo imaging system (IVIS) to trace transplantation of BMSCs, using Magnetic resonance imaging (MRI) T2series to scan the experimental mice. Finally processed all the MRI raw data using Philips DICOM Viewer R3.0image workstation and measured the left renal pelvis volume of the experimental mice at different time points, by comparing the left ureter recanalization rate after removing left ureteral obstruction of experimental group and control group mice to evaluate the repairmen effect of BMSCs on ureteral injury.Results:1.The number of BMSCs is linearly related to the intensity of Luc signal, BMSCs and PBS without Luc labeling showed no optical signals.2.The volume of the left renal pelvis in Laboratory mice became bigger obviously after left ureteral was obstructed (P<0.01), were the left renal pelvis volume of the experimental group and control group mice, had no statistical significance (P=0.693)10days after the left ureter obstruction was set up. At various stages of the mice of the left ureter obstruction after10days of the left renal pelvis volume had no statistical significance (P=0.583). Using microscopic vascular clamp can be used for setting up a balanced and stable left ureteral obstruction model in mice, the success rate is100%.3. Injecting BMSCs using30g insulin syringe, combined with medical biological fibrin glue for hemostasis can implement reliable transplanted BMSCs operation via the renal artery in mice, with the success rate of80%.4. At different time points after BMSCs transplantation, IVIS was used to scan the signals in experimental mice in vivo, specificial optical signals were visible in the left kidney area of experimental mice.10days after transplantation of BMSCs, IVIS was used for scanning experimental mice organs in vitro, optical signal was visible stronger in the left kidney and left ureter on the experimental mice, the optical signal could also be detaected in lung, liver, and left ovarian.5. In experimental mice, the left ureter recanalization rate is higher than that in the control group mice, after removing the left ureter obstruction (P=0.012)Conclusion:1. BMSCs transplantation through the renal artery is a kind of high efficient transplantation way to study the therapy effort of stem cells on ureteral obstructive disease treatment.2. Transplantation through the renal artery can promote the restoration of ureteral injury in mice.24Figures,8Tables,60References... |
PDF Full Text Request