Comparative Analysis Of Anti-Tumor Effect And HN Gene Of Newcastle Disease Virus HBNU/LSRC/F3, Mukteswar And Lasota Strain

Newcastle disease virus (NDV) belongs to Avulavirus genera of the Paramyxoviridae family, which are enveloped viruses containing single stranded negative-sense RNA. It was discovered and named in1927. NDV can infect a variety of human tumor cells, and specifically kill tumor cells. However, NDV is safe to normal human cells. Esophageal cancer is a highly invasive malignant gastrointestinal tumor with resistance to traditional radiotherapy, chemotherapy and surgery.The experiment studied the apoptotic effects induced by three NDVs (lentogenic HBNU/LSRC/F3strain, lentogenic LaSota strain and mesogenic Mukteswar strain) on human esophageal cancer ECA109cells. Groups of HBNU/LSRC/F3strain, Mukteswar strain, LaSota strain and negative control were set up in the experiment. CCK-8method, Laser confocal microscope, flow cytometry and agarose gel electrophoresis were applied to observe the apoptosis of EC A109cells infected by the NDVs. Designed specific primers were conducted to amplify HBNU/LSRC/F3strain HN gene sequences. Comparative analysis to the nucleotide homology of the3NDVs was performed. CCK-8method showed that ECA109cells proliferations were inhibited by the3NDV strains. Early apoptosis was determined by flow cytometry. Apoptosis rate of HBNU/LSRC/F3group was21.32±0.44%, Mukteswar was22.27±0.23%and LaSota was14.32±0.61%.The apoptosis was observed with Laser scanning confocal microscopy, either. The clear DNA ladders were observed in the DNA gel electrophoresis. Compared with3NDV strains, the homology of nucleotide sequences is from88%to91.8%, and amino acid is from90.2%to93.3%.HBNU/LSRC/F3strain may be the best one for antitumor therapy in the three strains.