The Role Of BDNF In The Pathogenesis Indueed By Nitroglycerin In A Rat Model Of Migraine

ObjectivesTo preliminarily explored the possible role of BDNF、TrkB、ERK、 p-CREB in the pathogenesis of migraine.MethodsFirstly, adult Sprague Dawley (SD) rats whose weight are180-220g were randomly divided into model groups and control groups. Secondly, we developed a rat model of nitroglycerin (NTG) induced to migraine. The ethology of rats in model group and control group was observed. Thirdly, we detected the level of BDNF by ELISA in serum and expression of BDNF、TrkB、ERK. p-CREB by means of RT-PCR and Immunohistochemistry in the trigeminal ganglion of migraine rats.Results①Ethology results showed:the data of ethology was revealed*compared with control groups, rats in model groups had significant painful behavior(P<0.05).②ELISA results showed that the expression of BDNF in serum in the period of onset of model groups, were significantly increased compared with in the period of intermission of model groups and in control groups (P<0.05), their gender difference were no statistical significance(P>0.05).③RT-PCR results showed the expression of mRNA of BDNF. TrkB、ERK1、p-CREB in the period of onset of model groups, were more than those in control groups and in the period of intermission of model groups(P<0.05), but no gender difference(P>0.05).④The results of Immunohistochemistry showed:the expression of BDNF、TrkB、p-ERK. p-CREB protein in the trigeminal ganglion in the period of onset of model groups, were significantly increased compared with in control groups and in the period of intermission of model groups(P<0.05), but there was no significant gender difference(P>0.05).ConclusionsAccording to the obtained results, the upregulated expression of BDNF、TrkB、ERK、p-CREB mRNA and protein may have a correlation with occurrence and development of migraine.