Anti-PDGFR-α Antibody Induction In Rust Role In The Process Of Human Retinal Pigment Epithelial Cell Proliferation

ObjectiveRust induction hRPE cells proliferate differentiation, PDGFR alphaantibody for inhibitors, research PDGFR-alpha antibody can effectively inhibithRPE cells proliferation and its mechanism.Methods1.In vitro hRPE cells-19, the exponential phase of cell experiment,Prussian blue staining to observe cell to gobble up the rust particles.2.Using0.1mg Fe₂O₃processing hRPE-19, different concentration ofPDGFR-α role in RPE-19, determined by MTT method is used to detect thesurvival rate of RPE cells.3.Experiment is divided into three groups: blank control group, rust group,the experimental group （rust particles plus1,10,50and anti PDGFR l00ug· ml-1-alpha antibody treatment）, respectively, in the role of0,12,24and48hours after determined by MTT colorimetric method is used to detect antiPDGFR alpha antibody influence on rust induced hRPE cells proliferation.4.RT-PCR to detect the expression of VEGF and TNF-α mRNA hRPEcells.Results1.Prussian blue staining to observe cell to gobble up the rust particleshave to consume ability.2.Trypan blue staining to detect hRPE cells survival were greater than80%.3.As PDGFR-αhRPE proliferation rate decreased, increased αconcentration of alpha for.4.Determined by MTT test of different duration PDGFR-αhPREproliferation, the influence of the concentration of PDGFR-αrole in differenttime after all the experimental hPRE cultivation hole with A value of0h wasstatistically significant difference were observed in the control groupcomparison（P<0.05）.PDGFR-α different concentrations of hRPE proliferation,the influence of all experimental hPRE cultivate hole with A value0μg/mLcomparative differences are statistically significant（P<0.05）.Experiments foundthat concentrations of50μg·ml-1PDGFR-α for the optimum concentration ofcell proliferation inhibition of hRPE cells.5.RT-PCR detection hRPE cells, anti-PDGFR alpha antibodies inhibitVEGF and TNF-αmRNA expression mechanisms to inhibit cell proliferation.Conclusions1.PDGFR alpha antibody can effectively inhibit hRPE cells proliferation invitro, and dose-response relationship was within a certain range.2.50ug. mI-1to curb hRPE cells proliferation is the most effectiveconcentration.3.PDGFR alpha antibody can make the cut, VEGF expression and TNF-αconfirmed PDGFR alpha antibodies inhibit VEGF expression and TNF-αmechanism is to suppress the proliferation of RPE cells.