Synthesis And DNA-binding Properties Of The Curcumin Derivatives Metal Complexes

Three of the curcumin derivative ligand （L^1L3） and two of their metal complexes（CuL₂²and CuL₂³） were synthesized. The chemical structure of the target ligand werecharacterized by infrared spectroscopy （IR）, proton nuclear magnetic resonancespectrum （1H-NMR） and mass spectrometry （MS）. The single crystal of ligands （L^1L3）and metal complexes （CuL₂²、CuL₂³）have been cultivated and its crystal structure wasdetermined by X-ray diffraction. The interaction of the metal complexes with ctDNAhas been investigated by using UV-Vis absorption spectra, cyclic voltammetric （CV）measurements and fluorescence spectroscopy. It can be seen that hypochromism wasobtained in the CuL₂³UV-Vis spectra, and that the redox peak current reduction andpeak potential shifted positively of the CuL₂³CV spectra with the increasingconcentration of ctDNA, but the position peak of the CuL₂²under the same conditiondidn’t changed significantly. The system of EB-DNA’s fluorescence intensity could beweakened by CuL₂³, but CuL₂³didn’t. The experiment results indicate the CuL₂³may bebind to ctDNA in an intercalative mode, and that of compared with the properties ofCuL₂³the CuL₂²was electrostatic binding mode.