Symphysial Detection Of Serum S-100B, NSE,GFAP And UCH-L1Was Helpful To Evaluate Severity And To Predict Outcome In Patients With Severe Traumatic Brain Injury

Objectives:The aim of this study was to investigate the significance of S100B protein (S100B), neuron-specific enolase(NSE),glial fibrillary acidic protein (GFAP) and Ubiquitin Carboxy terminal hydrolase1(UCHL1)biomarker concentrations in serum for severity and outcome evaluation after severe traumatic brain injury (TBI).Methods:In order to assess the increase of S-100B, NSE, GFAP and UCH-L1biomarker in serum(s-biomarker) after a severe TBI, we collected serum samples(s-S-100B. s-NSE. s-GFAP and s-UCH-L1) from46patients with severe TBI starting at the time point of12hours after the trauma, and then at the time point of24h,3d,7d and14d after the trauma, respectively. All specimen were measured using Enzyme Linked Immunosorbent Assay (ELISA). The severity was assessed in the first3days by Glasgow coma scale (GCS).The Glasgow outcome scale (GOS) assessed outcome after6months.Results:Patients with severe TBI had significantly(p<0.0001) higher initial s-S-100B. s-NSE, s-GFAP and s-UCH-L1values in the acute phase compared with members of the control group. There were no significant differences in the initial s-biomarkers levels of patients with gravis type TbI (GCS6-8) in comparison with patients with specific gravis type TBI (GCS3-5). Patients with unfavourable outcome had significantly(p<0.001) higher initial and late s-biomarkers values compared with patients with favourable outcome.Conclusion.Serum-biomarkers increased during the first days after a severe traumatic brain injury. They were predictions of TBI,but they didn’t show the ability to distinguish the gravis type and specific gravis type in TBI. Either initial or late values of the biomarkers are related to clinical outcome in TBI. GFAP and UCH-L1, especially, were superior predictions of TBI.