Curcumin Mitigates The Changes Of Cognitive Function By Sevoflurane Anesthesia In Aged Rats

PartⅠ The effects of anesthetic sevoflurane on cognitive function inold rats with different concentrationsObjective:To investigate the effect of different concentrations ofsevoflurane anesthesia on cognitive function in aged rats.Methods:Eighty male Sprague Dawley rats aged18months weighing490-590g were randomyly divided into five groups:0%sevoflurane group(C, n=16),1%sevoflurane group (S1, n=16),2%sevoflurane group (S2,n=16),3%sevoflurane group(S3,n=16) and4%sevoflurane group(S4,n=16); All rats received anesthesia for6h. The cognitive function was testedwith Morris Watermaze. APP, Aβ and BACE-1expressions in thehippocampus were determined with enzyme-linked immunosorbent assaysand Western-Blot after anaesthetized24hours.Result:1.Compared with the control group, both latency and the number ofseeking for hidden-platform has significant difference in S3and S4groups (p<0.05),but no significant difference among the S1and S2groups(P>0.05).2.The expressions of APP and Aβ in the hippocampus was increasedwith increasing concentrations(p<0.05).3.Compared with the control group,the expression of BACE-1inhippocampal was significant increased in S3and S4groups (p<0.05).but nosignificant difference among the S1and S2groups(P>0.05).Conclusions:1. The effects of sevoflurane on cognitive function in aged ratsdepends on concentration, the higher concentration(>3%) of sevofluranethe stronger.2.Effect of high concentration of sevoflurane on cognitive function,Possibly by inducing APP, A β generation and caused up-regulation ofBACE-1.Part Ⅱ Curcumin mitigates cognitive function impairment inducedby sevoflurane anesthesia with high concentration in aged ratsObjective:To investigate the curcumin effect on cognitive functionafter sevoflurane anesthesia with high concentration and the expressionof Cyclooxygenase-2in aged ratsMethods: Forty-six male Sprague Dawley SD rats aged18months weighing500-600g were randomyly divided into three groups: Blankcontrol group (C, n=16),3%sevoflurane group (F, n=16),3%sevofluraneand curcumin group (F+H, n=16); F group and (F+H) group receivedanesthesia for6h. The cognitive function was tested with Morris Watermaze.Aβ, Cox-2and BACE-1expressions in the hippocampus were determinedwith enzyme-linked immunosorbent assays and Western-Blot.Result:1.Compared with the control group, both latency and the number ofseeking for hidden-platform has significant difference in H and (H+F)groups (p<0.05),there has significant difference among the H group and(H+F)group(p<0.05).2.Compared with blank control group, the expression of Aβ in thehippocampus has significant difference in F group (p<0.05),but nosignificant difference among the C and (F+H) groups(P>0.05).3.Compared with the control group,the expression of BACE-1inhippocampal was significant increased in F and (H+F) groups (p<0.05).4.There has no significant difference among the H group and(H+F)group and C group(p<0.05).Conclusions: Curcumin might mitigates cognitve function impairmentinduced by sevoflurane with high concentration.Possibly by suppress APP,A β generation and caused down-regulation of BACE-1.