The Role Of CD39in The Propranolol Treatment For Proliferating Hemangioma In Nude Mice

objective: To evaluate the relative mechanism of CD39inpropranolol treatment for proliferating hemangioma by hemangioma model.Methods: Hemangioma model in nude mice was established by implantingthe pieces of proliferating hemangioma into the subcutaneous areas of nudemice. Sixty live implanted nude mice were randomly divided into twogroups for experiment: Group1(30mice), intervention group withpropranolol; Group2(30mice), control group with physiological saline.There were fifteen mice in each group and there were two pieces ofimplanted tumors in each nude mouse. Two groups were carried out lavageintervention treatment once every other day by being given propranololsolution and physiological saline respectively. The volume changes of eachhemangioma tumor in nude mice were measured respectively before andafter treatment to observe the hemangioma’s growth. On the first day withthe first time intervention, and in one week, two weeks and three weeks afterintervention, The nude mice were killed by dislocating cervical vertebrae fortest at different time points(before intervention,7thday,14thday,21thdayday and28thday afer intervention). Then the implanted tissues were resectedto make into slices for histologic analysis with HE stain. At the same time,the expressions of CD39, Caspase-3and Ki-67were carried out withimmunohistochemical staining. Apoptosis status of the transplanted tissue was detected in TUNEL method. ATP concentration was measured byELISA method with fresh samples. All the experimental data were analyzedby statistics with SPSS19.0statistical software. Results:1. Before theintervention, the growth of implanted hemangiomas in each nude mouse wasgood, and the volume had no significant difference between the two groups（P＞0.05）. After intervention, the volume of hemangioma in propranololgroup was smaller than that in saline (P＜0.01).2. Under microscopy, inpropranolol group, vascular vessels of tumors reduced, fibrofatty tissuesinfiltrated, cells scattered sparsely and atrophied, vessel walls collapsed anddeformed, and lumen occlusion and destruction occurred; While in the salinesolution group, the vessels were rich, with no destruction of vessel walls andlumen, showing a proliferating change. The optical density of CD39and thepositive index of Ki-67in propranolol group were lower than those in萨里呢solution（P<0.05）; Caspase3and the apoptosis index in propranololgroup were higher than those insaline solution group（P<0.01）. The ATPconcentration of propranolol group was higher than that of saline solutiongroup（P<0.05）. Conclusions: Propanolol can restrain the growth ofhemangioma and promote its involve. The mechanism of propranolol fortreating proliferating hemangioma may has relationship with the expressionchange of CD39which can affect the development of hemangioma throughthe regulation of ATP.