Application Study On Extraction Of Active Ingredients In Ginkgo Biloba Leaf By High Intensity Pulsed Electric Fields

Objective: the flavonoids and ginkgolides of Ginkgo biloba Linn. were extracted byhigh intensity pulsed electric fields (HPEF) and tested by HPLC, and process parameters ofadsorption test were optimized, and the paper can provide theoretical basis forcomprehensive development and industrial production.Methods: the flavonoids and ginkgolides of G. biloba were tested by HPLC-UV andHPLC-ELSD, respectively. The effects of four factors, such as solid-liquid ratio, electricfield strength, pulse number, on falconoid and terpene lactone extraction of G. biloba wasstudied by orthogonal test. The adsorption and elution capacity of five kinds of resin D101,AB-8, DM130, HPD417, ADS-17for flavone and lactone of G. biloba were alsoinvestigated.Results: the result showed that the factors on falconoid extraction was the order ofliquid ratio, electric field strength, pulse number, extraction solvent, The optimumextraction process that the electric field strength was15kV/cm, the pulse number was14,the ethanol concentration was70%, the solid-liquid ratio was1:12; and the factors onterpene lactone extraction was the order of electric field strength, pulse number, extractionsolvent, ratio of solid to liquid, the optimum extraction process that the electric fieldstrength was15kV/cm, the pulse number was12, the ethanol concentration was70%, thesolid-liquid ratio was1:12. The time was1/30of ultrasonic extraction and1/120of ethylalcohol reflux; and the solvent consumption was less, the amount of solvent of HPEF was1/4of ultrasonic extraction and1/5of hot alcohol reflux extraction, the process wasfeasible by certifying test.The adsorption and elution capacity of DM130for flavonoids and ginkgolides of G.biloba were the best, and the adsorption of flavonoids and ginkgolides of G. biloba were14.4mg/mL and6.7mg/mL. Water washed off the volume was8BV, ethanol concentrationof70%washed off volume was6, adsorption rate was2BV/h, wolf velocity was1BV/h.The ginkgolides concentration in different eluent was different using differentconcentrations of elution,80%ethanol should be use if the objective was extraction and purification of lactone.Conclusions: The results can provide theoretical basis for quickly extraction andseperation and purification of flavonoids and ginkgolides of G. biloba.