The Role Of XPD In Hepatoma Cell Growth And ERG Gene Expression In Vitro

Objective：To discuss the effects of transfected XPD gene on the growth ofhepatoma cell SMMC-7721and the expression of ERG gene in the cells in vitro.Methods: The XPD gene was transfected into human hepatocellular carcinomacells SMMC-7721with Lipofectamine2000in transient transfection. Experimentwas divided into four groups, respectively, containing recombinant plasmidtransfection liver cancer cells SMMC-7721-pEGFP-N2-XPD group (XPDgroup),empty plasmid transfection liver cancer cells SMMC-7721-pEGFP-N2group (N2group),liposome transfection liver cancer cells SMMC-7721group (Lipgroup) and liver cancer cells SMMC-7721(blank control group). We usedRT-PCR and Western blot to measure the expression levels of XPD and ERG mRNAsand proteins, respectively,in four groups of hepatoma cells. The cell proliferation wasassessed by MTT and the cell apoptosis was detected by flow cytometry instrument infour groups of hepatoma cells.Results: Compared with other three groups, the expression levels of EGRmRNAs and proteins were obviously declined in XPD group (P <0.01), while theexpression levels of XPD mRNAs and proteins were elevated significantly in XPDgroup (P <0.01). The proliferation of cells was decreased significantly and the cellapoptosis rate was increased obviously after transfection of XPD (P <0.01).Conclusion: The wild-type XPD can inhibit the expression of ERG,decrease theproliferation of SMMC-7721cells as well as enhance the apoptosis of the cells invitro.