| Objective：Investigate the expression of Akt1, Girdin and ARK5in breast cancer andexplore the relationship between their expression and clinicopathological parameters.At the same time, explore the relationship between of Akt1, Girdin, ARK5andmetastasis and prognosis of breast cancer, study if these three antibodies can predictthe prognosis of patients and to assess the prognosis.Methods:88cases of breast cancer and10cases of cancer-adjacent normal tissues wereabtained from Jiangxi Provincial People’s Hospital between2006-01-01and2010-12-31. By tissue microarray method, all breast cancer samples were embeddedinto three paraffin blocks. The protein expression of Akt1, Girdin and ARK5weredetected on the paraffin sections by Envision two-step immunohistochemiscal method.The relationship between their expression and clinicopathological features wereanalysised.52cases had follow-up date, using telephone to find out patients’ survivalsituation and making survival analysis (Follow-up time until March31,2013, ordeath date, or，lost date). We studied the relationship between the three proteinexpression (Akt1, Girdin and ARK5) and clinicopathological parameters andevaluated patients’ prognosis. When we analyzed the expression differences of Akt1or Girdin, we used the Kolmogorov-Smirnov Z test between the two groups ofclinicopathological parameters and used Kruskal-Wallis H test between the threegroups. The expression differences of ARK5in the clinical and pathological featureswere used Chi-square test or Fisher’s exact probability test. Non-parametricSpearman rank correlation analysis was used to two factors analysis. Univariateanalysis was performed using the Kaplan-Meier method and the Log-Rank test. Weevaluated the three proteins and the clinicopathological features for assessment ofprognosis. We made multivariate survival analysis by COX proportional hazardsregression model. Results：(1)In88cases breast cancer, the positive expression rates of Akt1, Girdin was77.3%(68/88) and80.7%(71/88), high expression rate of ARK5was48.3%(43/88).In10cases of cancer-adjacent normal tissues, the positive expression rates of Akt1,Girdin was30.0%(3/10) and40.0%(4/10), high expression rate of ARK5was20.0%(2/10). The expression of Akt1and Girdin in breast cancer were higher than inadjacent normal tissues (P<0.05), while, there was no difference between breastcancer and adjacent normal tissues about espression of ARK5(p>0.05).(2) In the allcases of breast cancer, the expressions of Akt1were different in lymph nodemetastasis and pathological staging (P<0.05), but the expressions of Akt1in otherclinicopathological parameters had no differences(P>0.05); the expressions of Girdinwere different in various sex and lymph node metastasis (P<0.05), but the expressionsof Girdin in other clinicopathological parameters had no differences(P>0.05); theexpressions of ARK5were different in lymph node metastasis, lymph node metastasisnumber, tumor diameter(P<0.05), but the expression of ARK5in other clinicopatholo-gical parameters had no differences(P>0.05).(3)Non-parametric Spearman rankcorrelation analysis showed that: there was a positive correlation with Akt1andGirdin(r=0.308, P=0.004); and a positive correlation with Akt1and ARK5(r=0.343, P=0.001), while there were no correlations between Girdin and ARK5(p>0.05).(4) The expression of Akt1, ARK5and expression of ER, PR, cerbB-2, p53,Ki-67had no differences (p>0.05). The expression of Girdin and expression of ER,PR were significantly difference (p<0.05), while the expression of Girdin andexpression of cerbB-2, p53, Ki-67had no differences (p>0.05).(5) In follow-up dataof54patients, there was no difference between three antibodies expression andcumulative survival rate.(6) Univariate survival analysis demonstrated that: therewere significant differences in median survival time according to lymph nodemetastasis, lymph node metastasis number and pathological staging (P<0.05). COXmultivariate regression model showed that: only lymph node metastasis number（p<0.05）are the independent prognostic factors influencing prognosis of breastcancer patients. Conclusions：(1)There was a correlation between expression of Akt1，Girdin and ARK5inclinicopathological parameters of breast cancer, especially in the lymph nodemetastasis.(2) The expression of Akt1and Girdin in breast cancer was higher than inadjacent normal tissues, and three proteins expression in breast cancer have somerelationship between each other.(3)There was highly positive expression of Akt1andGirdin, which suggested that these factors were concernerd with the development andprogression of breast cancer.(4) Akt1，Girdin and ARK5were not independent factorsof clinical outcome in breast cancer. |
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