The Lung Protective Effects Of Ulinastatin On Acute Lung Injury Rat Model

Object: To evaluate the effects of ulinastatin(UTI) on expression of TNF-a mRNAIL-I mRNA,and IL-lra mRNA in acute lung injury(ALI).Methods:72male Sprague Dawley rats Application random number table methodis divided into4groups,the experimental control group (A),the injection of endotoxingroup(B) and ulinastatin low-dose group(C50ku kg-1),the the ulinastatin high-dosegroup(D100ku kg-1)，30minutes prior to the model give to group C and D rats byintraperitoneal injection of different doses of ulinastatin.The BCD group wereused intravenous injection of endotoxin phosphate lipopolysaccharide (LPS) cause acutelung injury model.The A group intraperitoneal and intravenous injection of the sameamount of saline. After injected lipopolysaccharide(LPS)2hours6hours and24hours,catch each group sampling6; The rats supine fixed on the console，used1%sodium pentobarbital (50mg/kg) intraperitoneal injection to anesthesia，open-chestlung,10%formalin-fixed the lung tissue of right lower lobe until pathologicalexamination. Right lower lung tissue frozen at-80°C,HE staining,light microscopy oflung tissue changes,the primer primer5.0of the application of primer design softwaredesign primer purchased primer,reverse transcription polymerase chain reaction(Reverse Transcription-Polymerase Chain Reaction， RTPCR) assay in rat lung tissueTNF-α mRNA the IL-1βmRNA， IL-1ra mRNA expression. Detection of differentdoses of ulinastatin under intraperitoneal injection of endotoxin lipopolysaccharide(LPS) induced acute lung injury in rat lung tissue TNF-α,IL-1β,IL-1ra mRNAexpression.Results： the lung tissue of rats by HE staining， the control group rat alveolartissue structure is more clear and complete destruction of the interval withoutbroadening endotoxin lipopolysaccharide (LPS) in lung tissue inflammation,varying degrees of alveolar structure by loss,alveolar tissue septal thickening wider,inflammatory cell infiltration,amount ulinastatin group compared with endotoxin thanto show some suppression of inflammation,alveolar tissue interval narrowing relieveinflammatory cell infiltration; on of TNF-α mRNA，IL-1βmRNA,mRNA expression ofIL-1ra,application rtPCR and RNA in the lung tissue of lung injury in ratssemi-quantitative analysis of the results of statistical analysis after SPSS19softwareapplication analysis of variance and t tests， respectively,the same as different timepoints treated TNF-α expression differences between the groups,the results suggest thatthe statistically significant difference between ulinastatin intervention group withendotoxin group (P <0.001), the ulinastatin low-dose group and high-dose grouprelative difference is significant (P <0.001),to compare the same treatment group ofbetween-group differences， the difference was no significant,Similarly,comparison ofIL-1β and of IL-1ra expression of the case,application of the Ukrainian Division hissm-all group and within toxin group showed a significant difference (P <0.005).Conclusions： UTI can decrease the levels of TNF-α IL-1β and IL-1raexepression in LPS induced ALI of Sprague Dawley rats in a dosage dependentmanner and has the role of regulation of the immune balance.