The Expression Of UPA Ang UPAR In Pancreatic Cancer And Its Clinical Application

Background:Tumor recurrence and metastasis are the most importantreasons why the motality rate of patients with malignant is high.Themetastasis is not a random process,different tumor cells metastasis tospecific tissues and organs.And patients always die from this.Effectiveprevention and early diagnosis and treatment become the research focus incancer treatment including pancreatic cancer.uPA and uPAR reflect theprogression and biological characteristics of pancreas tumor.Currentresearch suggested that the key step of pancreatic cancer metastasisinvolves activation of malignant proteolytic enzymes and the degradation ofextracellular matrix components.The system of uPA may play a positive rolein this process.Overexpression of uPA may be associated with tumor cellinvasion and metastasis and affect the pathological grade of malignant.There is a great importance in the treatment and diagnosis of pancreaticcancer to learn more about uPA and uPAR.Purposes:The research of inhibition of uPA and uPAR is always the hot potin the clinical anti-tumor therapy,however this inhibition is often limited bythe location,pathological type and clinical stage.Thus this experiment wasdone at different levels of organization and protein to observe theexpression of uPA ang uPAR in pancreatic cancer.And it provided newtheoretical basis and clinical ideas for the non-surgical treatment andcomprehensive treatment of pancreatic cancer.Method: Pancreatic cancer with no athymic nude mice modelpreparation:injected BXPC-3pancreatic cancer cell lines into the left andright armpit subcutaneous of mice.One month later the tumor increased toabout1.0×1.0cm.It meaned the models were successfully. The technetiumsalt labeled tumor imaging:using immunohistochemistry and western bloting detected uPA and uPAR expression in pancreatic cancer tissue.Result:Use Tricine as collaborative ligand and SnC12as reducing agent.Radioisotopes(99Tcm-Hynic-PEG-AE105) marked the tumor which wasinoculated in mice,then analyzed the imaging results.Analyzed theexpression of uPA and uPAR by immunohistochemical techniques andwestern blotting.There was no correlation between the expression of uPAand uPAR and the patient`s age,sex and the location,size,degree ofdifferentiation of the tumor.(P>0.05)Conclusion:Tumor develop significant after injection99Tcm-Hynic-PEG-AE105.uPA and uPAR proteins express highly inpancreatic cancer.And the expression of uPAR was positive correlation withthe ingest of99Tcm-AE105in4-6h.The purpose of inhibiting tumor can beachieved by inhibiting the protein uPAR.