| Objective:To investigate the effects of triptolide on the permeability of blood brain barrier and protection of neurons during cerebral inflammation induced by LPS.Methods:Healthy adult male SD rats were randomly divided into3groups:Normal saline group(NS+NS, n=10),LPS group (LPS+NS, n=10),Triptolide group (LPS+T10,n=10).Nissl staining,GFAP immunohis-tochemical (IHC) staining and Evans blue fluorescence tracer method were used in the present study.Results:The results of Nissal staining showed that the pyramidal neurons in the hippocampal CA1region of rats in the NS+NS group arranged normally and no remarkable neuronal loss. In LPS+NS group, both the density and the layers of pyramidal neurons was less than that in LPS+T10group and NS+NS group. The neurons in the hippocampal CA1region of the rats in LPS+NS group were scattered randomly and the intercellular spaces increased.GFAP IHC staining shown that GFAP positive cells were distributed sparsely in the hippocampal CA1region of the rats in the NS+NS group, which had smaller cell bodies and slender processes, and also were stained more lightly. The number of the positive cells increased more obviously in LPS+NS group and LPS+T10group compared to that in NS+NS group. The number of astrocytes increased,the cell bodies and processes were bigger, and the color of the stained cells was darker in LPS+NS group than those in LPS+T10group. During the Evans blue fluorescence staining, showed that the intensity of EB fluorescence in cerebral cortex and hippocampus formation as wall as the cerebral vessels, higher than that in LPS+T10group and NS+NS group. However, there was no significantly statistical difference between the results of EB fluorescence staining of T10+LPS group and NS group.Conclusion:The study suggests that T10may influence the function of BBB and reduce the inflammation reaction of astrocytes to protect the neurons from impairment during cerebral inflammation process induced by LPS. |
PDF Full Text Request