The Effects Of Bone Marrow Stromal Cells On The Proliferation Of Multiple Myeloma Cells And Intervention With Lentinan

objective:Through separating bone marrow stromal cells from multiple myeloma patients and controls, establishing the BMSCs and U266cells co-culture system, with MTT assay and flow cytometry, we detect the differences of membranous BAFF(CD257) expression intensity between BMSCs from MM patients and controls, analysis the effects of BMSCs on the proliferation of multiple myeloma cells with lentinan intervention.Methods:In this study, we isolated and cultured BMSCs from bone marrow of12previously untreated MM patients and5controls, then detected the CD257expression intensity on the two kinds of BMSCs with flow cytometry. We cultured U266cells with the conditional medium derived from MM patients and controls, then analysised the proliferation of U266cells by using MTT assay. Established BMSCs derived from MM patients and U266cells co-culture system, which was interfered by lentinan, then detected CD257expression intensity of BMSCs with flow cytometry, and analysised the U266cells survival and proliferation by MTT assay. From that we can explore the effects of BMSCs on the proliferation of multiple myeloma U266cells.Result:Increased CD257expression level on BMSCs derived from MM patients compared with the controls(P<0.05).Both conditional medium derived from the two kinds of BMSCs can promote the proliferation of U266cells, There is significant difference compared with serum-free medium group, but there is no significant difference between MM patients group and the control group. It revealed that BMSCs can promote the proliferation of myeloma cells maybe through the secretion of cytokines. With flow cytometry, we observed that after lentinan interfering BMSCs and U266cells co-cultured system,CD257expression intensity on BMSCs was decreased. Aslo with MTT assay, it is show that lentinan inhibit the proliferation of U266cells more obviously in BMSCs and U266cells co-culture system than in U266culture alone without BMSCs support. Conclusion:Our study show that, by isolating and culturing BMSCs derived from MM patients and the controls, establishing BMSCs and U266cells co-culture system, with MTT assay and flow cytometry,we can conclude that increased CD257expression level on BMSCs derived from MM patients compared with the controls, BMCSs of MM patients promote the survival and proliferation of U266cells. At the high concentration, lentinan can inhibit the proliferation of myeloma U266cells maybe through interfering the interaction of BMSCs and U266cells except direct inhibiting the proliferation of U266cells weakly.