The Cytotoxicity Of Paclitaxel-silica Nanoparticle To Taxol-resistant Nasopharyngeal Carcinoma Cells

Objective:l.To explore the paclitaxel injection combined with the silica nanoparticles in vitro.2.To detect the toxicity of the silica nanoparticles on nasopharyngeal carcinoma cell, determine the safe concentration range.3.To compare the paclitaxel silica nanoparticles with paclitaxel injection on the sensitivity for the nasopharyngeal carcinoma cells.Methods:1. Mixed different concentrations of paclitaxel injection with different concentrations of silica nanoparticle solution and use different concentrations of paclitaxel alone as a control. After centrifugal, take supernatant for high-performance liquid chromatography (HPLC), and determine the free paclitaxol concentration, and the combination rate of paclitaxel and silica nanoparticle determined on pure paclitaxel concentration divided by free paclitaxel concentrations;2.Use the colony formation test to detect the cytotoxicity of the silica nanoparticles to CNE1、HNE2、5-8F nasopharyngeal carcinoma cells and their’s drug resistant cells.3. Fix the silicon nanoparticle concentration getting from the second test that upper limit concentration from the silicon nanoparticle silica nanoparticle concentration range of the security for the nasopharyngeal carcinoma cells, and set the group which didn’t add silica nanoparticles as control group, and set the group that add silica nanoparticles as test group, also utilize the colony formation test,to compare the colony numbers from the different concentrations of the control group and test group on CNE1、HNE2、5-8F parental cells and their’s drug resistant cells, and then calculate the colony forming inhibition rates, to compare their ability to resist nasopharyngeal carcinoma cells with their different colony forming inhibition rates. Results:1.The results from the high-performance liquid chromatography (HPLC) show that the combination rate of nanoparticles and paclitaxel gradually increased following an increase of the ratio of silica nanoparticles/paclitaxel concentration. When the silica nanoparticles is1000times than that of the paclitaxel concentration, the combination rate is21.3%.2.The colony formation test results showed when the silica nanoparticles on nasopharyngeal carcinoma cell is0-10ug/ml, the cell inhibition rate is0.3.The clony formation test result showed the control groups, when both cells lines treated by taxol, the IC50value of CNE1and CNE1/taxol is respectively1.10±0.07ng/ml,9.55±0.24ng/ml; the IC50value of HNE2and HNE2/taxol is respectively0.93±0.07ng/ml,7.64±0.22ng/ml; the IC50value of5-8F and5-8F/taxol is0.81±0.07ng/ml,6.43±0.24ng/ml.The test groups, when both cells lines treated by paclitaxel and silica nanoparticle, the IC50value of CNE1and CNEl/taxol is respectively0.87±0.07ng/ml,8.35±0.21ng/ml; the IC50value of HNE2and HNE2/taxol is respectively0.86±0.07ng/ml,6.75±0.21ng/ml;the IC50value of5-8F and5-8F/taxol is respectively0.77±0.07ng/ml,5.99±0.21ng/ml. Using statistical analysis, compare with that test groups adding the silica nanoparticles with that control groups no adding silica nanoparticles, there is the statistical significances in the IC50values of six kinds of cells.Conclusions:1. The silica nanoparticles can combine with the paclitaxel in vitro.2. When the concentration of silica nanoparticles is0to10ug/ml, it is relatively safe for nasopharyngeal carcinoma cells.3. The silica nanoparticles can enhance the sensitivity of paclitaxel on the nasopharyngeal carcinoma cells.(8figures,31references)...