The Study Of The Influence Of HBV Infection On The Immune Function Of T-Lymphocyte

[Purpose]The hepatitis B has a complicated pathogenesis. It is currently considered that the disorder of the immune function is the main mechanism of the pathologic lesion. Accumulated evidences show that the cellular immune response is involved a pathogenesis of hepatitis B infection. While T-lymphocyte is the main immune cells and plays an important role in immune system. The abnormal proliferation and dysfunction of T-lymphocyte subsets contribute to the liver tissue lesion in the patients with HBV infection. The aim of this study was to determine states of cellular immune response in the patients with various types of hepatitis B, and the relationship among HBeAg, HBV-DNA quantities, YMDD variation and different genotypes and the clinical significance by detecting the absolute number of CD3+、 CD4+and CD8+of peripheral blood T-lymphocyte subsets and the changes of the ratio of CD4+/CD8+in hepatitis B patients with FCM.[Methods]1. Flow cytometry(FCM) was used to detect the absolute number of CD3+、 CD4+、CD8+and the ratio of CD4+/CD8+of T-lymphocyte subsets in peripheral blood of228hepatitis B patients and the normal control group.2.Electrochemiluminescence immunoassay(ECLIA) was used to detect HBsAg、anti-HBs、HBeAg、anti-HBe and anti-HBc in peripheral blood of the aforementioned228patients and to divide them into HBeAg (+) group and HBeAg (-) group.3. Fluorescence quantity of real-time PCR was used to quantify HBV-DNA in peripheral blood of the same228patients and to divide them into HBV-DNA (+) group and HBV-DNA (-) group.4. Using Fluorescence PCR to detect YMDD variation in HBV-DNA (+) group and to divide them into YMDD variation group (include YVDD and YIDD variation) and YMDD wild group.5. Using Fluorescence PCR to detect genotype of HBV-DNA (+) patients with CHB. [Results]Compared with the normal control group, the results as follows:1. The absolute number of CD3+、CD4+and CD8+of peripheral blood T-lymphocyte subsets in AHB patients increases dramatically. But it decreases successively in patients with CHB、LC and PHC, especially in LC and PHC groups. However, the ratio of CD4+/CD8+of AHB、CHB、LC and PHC groups is a growing tendency.2. The absolute number of CD3+、CD4+and CD8+of HBeAg (+) group and HBeAg (-) group drops respectively. And the ratio of CD4+/CD8+grows in both groups, especially in HBeAg (+) group.3. The absolute number of CD3+、CD4+and CD8+of HBV-DNA (+) group and HBV-DNA (-) group drops respectively. And the ratio of CD4+/CD8+also grows in both groups, especially in HBV-DNA (+) group.4. The absolute number of CD3+、CD4+and CD8+of YMDD wild group、 YVDD variation group and YIDD variation group drops respectively in HBV-DNA (+) patients. Similarly, the ratio of CD4+/CD8+grows among the three groups and especially in the two variation groups.5. There is a decreasing tendency of the absolute number of CD3+、CD4+and CD8+of CHB patients with C genotype and B genotype. The same as the groups aforementioned, the ratio of CD4+/CD8+grows in the two groups and especially in C genotype group.[Conelusion]The results indicated that the imbalance of T-lymphocyte subsets and disorder of cellular immune function exists in hepatitis B patients.1. The cellular immune function increases in AHB patients. While the cellular immune function decreases in patients with LC and PHC.2. The cellular immune function of HBeAg(+) lower than that of HBeAg(-) in hepatitis B patients.3. The cellular immune function of HBV-DNA(+) decreases obviously in patients with YMDD variation.4. It is a decreasing tendency of the cellular immune function in C genotype and B genotype CHB patients, especially in C genotype patients.