Antitumor Activities Of The Nanoparticles Of Epigallocatechin Gallate-β-Lactoglobulin In Various Cancer Cell Lines

(-)-Epigallocatechin gallate (EGCG) is the most abundant catechin in green tea, and has the strongest antitumor activity among catechins. Howerver, EGCG is unstable in adversed temperature, light and pH conditions, which reduces its biological availability in vivo. These deficiencies limit its application in anticancer field. Nanotechnology can packet EGCG inside, which not only greatly improves the stability of EGCG to prevents it from the destruction of body fluids and the enzyme, but also let EGCG reach the tumor successfully and enter cells to produce a pharmacal effect since its own unique targeting principle and small size. β-Lactoglobulin (β-LG), one of animal whey proteins, is harmless to human body with good biocompatibility and can be used as nano carrier of EGCG.On the basis of our laboratory’s Research findings, using β-LG as the carrier of EGCG, four EGCG-β-LG nanoparticles (ELNP1, ELNP2, ELNP3, ELNP4) were prepared by thermal induction. The zeta potential, size and shape of the four nanoparticles were measured using the zeta potential instrument and the transmission electron microscopy, regard EGCG as the control group, using the MTT method to study four nanoparticles’s inhibitory activity on A375human melanoma cells, SPC-A-1human lung adenocarcinoma cells, BxPC-3in situ pancreatic adenocarcinoma cells, TE-1human esophageal cancer cells, MSTO-211H human lung cancer cells, NCI-N87human gastric carcinoma cells,4T1mice breast cancer cells, SK-OV-3human ovarian cancer cells,769-P human renal cell carcinoma cells, Hela human cervical carcinoma cells,22RV1human prostate cancer cells, B16mouse melanoma cells, C-33A human cervical carcinoma cells and CACO-2human colorectal cancer cells under different concentrations(0.2mg/mL、0.4mg/mL、0.6mg/mL) after treatment of24h,48h,72h and96h.The results showed that the diameters of four kinds of nanoparticles were20-30(nm), and their Zeta potential values were less than-30(mV), which indicated the four nanoparticles were stable. MTT results showed that the same nanoparticle had different inhibitory activities on different tumor cells, the different nanoparticles had different inhibitory actions on the same tumor cells, and among these nanoparticles, some of them have a better antitumor activity than free EGCG, compared with the the same dose of free EGCG, inhibitory effect of ELNP2and ELNP3on the tumor cells are better, the sensitivity of four kinds of nanoparticles on SPC-A-1human lung adenocarcinoma cells, TE-1human esophageal cancer cells, MSTO-211H human lung cancer cells, SK-OV-3humanovarian cancer cells, Hela human cervical carcinoma cells, B16mouse melanoma cells are stronger than other tumor cells, the nanoparticles which have stronger sensitivity have a better effect on inhibiting the tumor cells than the free EGCG.This study provides scientific basis for the application of these EGCG-β-LG nanoparticles in anticancer field.