The Effection Of Xyloglucan On HepG2Cells As A New Extracellular Matrix

Objective The scaffold provides a framework and initial support for the cells to attach, proliferate and differentiate, and form an extracellular matrix (ECM) in tissue engineering. Many research works have been done on this area. In this study, xyloglucan (XG) was used as a new synthetic extracellular matrix (ECM) for HepG2cell attachment in alginate (AL) capsules.The aim of this study was to test the effects of XG on HepG2cells adhesion and the albumin secretion, ammonia elimination, cell proliferation and Cx32, E-cadherin gene expression of encapsulated HepG2cells. Methods BCA method was used to measure the adhesion ratio of HepG2cells onto XG coated and uncoated cell culture plate surface. Human Serum Albumin Elisa kit and Ammonia Assay Kit were used to measure the albumin secretion rate and the ammonia elimination rate of encapsulated HepG2cells, respectively. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the gene expression of Cx32and E-cadherin in encapsulated HepG2cells. Results The adhesion ratios of HepG2cells onto coated surfaces gave a rising tendency and the cell number increased when the concentration of xyloglucan increased, indicating the promotion effects of xyloglucan on cell-matrix interactions and cell proliferation. The rates of albumin secretion and ammonia elimination were highest at1mg/mL XG, and higher than those of blank groups, respectively, suggesting the promotion effects of xyloglucan on liver-specific functions of encapsulated HepG2cells. The Cx32and E-cadherin genes in AL/XG capsules were more rapidly expressed, than in AL ones, respectively. These results showed that the multicellular spheroid formation of HepG2cells can enhance the liver-specific functions in the three-dimensional space in the presence of XG as a new synthetic ECM.