Expression Of PDGFR-β In Human Glioma And Clinical Significance

Objective: Glial cell tumors usually referred to as gliomas, also calledneuroectodermal tumors or neuroepithelial tumors, because it often occurs inneuroectodermal is the highest incidence of central nervous system tumors,with a high incidence of characteristics, the mortality rate is high. In clinical,histological appearance and pathological features are important factors reflectthe degree of malignancy, and can also help patients choose the appropriatetreatment and prognosis of the disease. According to the World HealthOrganization (WHO) classification of tumors of the nervous system: I do nothave atypia grade of lesion/low proliferative activity, often by surgicalresection can be cured; Once the tumor lesions showed infiltrative growth hasbeen designated as Class II or higher; grade III tumor cell division activityoften accelerates and nuclear atypia/anaplastic obvious; IV stage is a fatalmalignancy grade, tumor cells often infiltrated into the tissue surrounding thetumor, active mitotic activity, characteristic necrosis and extensiveangiogenesis. Gliomas and further relates to various tumor-derived growthfactor and its receptor, and with the progressive activation and inactivation oftumor suppressor genes oncogenes are closely associated with a variety offactors and a plurality of steps of the evolution of the process.PDGFR-β is an original such oncogene c-sis encoded promotingcytokines, overexpression of both PDGF and the receptor in a variety of tumorcells, which can stimulate c-myc, c-fos and more oncogene species generatedin the level of transcription of some cells also have a negative regulatory role.A common characteristic of malignant glioma is active RTK signaltransduction pathway is the most common EGFR mutations/amplification orPDGFR amplification/overexpression caused.Tumor angiogenesis and metastasis survival prerequisite, especially the prognosis of many human malignancies angiogenesis and disease, as wellas the length of survival, there are some positive correlation. Assessmentgenerate blood vessel, and the prognosis of malignant tumors of the body isone of the most critical tumor microvessel density (MVD) indicator.In this study, the normal brain tissue，different levels, different genders,different ages glioma tissues PDGFR-β and glioma microvessel density (MVD)study in order to explore the PDGFR-β occurred in glioma development role,providing a new method and theoretical basis for the treatment of glioma.Methods: Immunohistochemistry (IHC SP method) and real-timequantitative PCR (RT-PCR), together with HE staining,12cases were detectedin normal brain tissue due to high intracranial pressure, and69cases ofdecompression required after surgical resection and pathology has beenidentified in human glioma (glioblastoma tissue in all wHO classification as astandard, the section I, II grade tumors identified as low-level group, thesection III, IV grade tumors identified as high-level group), SPSS16.0statistical software to perform the appropriate statistical analysis of the resultsof the experiment. The same between the different indicators used to comparethe clinical and pathological types Kruskal-Wallis H test, the relationshipbetween the expression of PDGFR-β and MVD using Spearman rankcorrelation analysis, test level a <0.05.Results:1IHC and HE showed: in normal brain tissue and various levels ofhuman glioma tissues were detected PDGFR-β expression and MVD in lowlevels, and normal tissue found: uniform cytoplasm, nuclear non-atypia,vascular no significant change was found in the high-level organization: cellsincreased significantly, and a large, but less cytoplasm, especially atypiaparticularly evident, many tumor cell aggregates, as well as flower ringstructure, tumor microvessel density proliferation, endothelial swelling,irregular wall. Differences in levels of PDGFR-β expression in normal braintissue and various levels of human glioma tissue with statistically significant(χ2=62.663P=0.000<0.05), MVD in normal brain tissue and people at all levels differences in expression levels of glioma tissue with statisticallysignificant (χ2=59.946P=0.000<0.05), PDGFR-β associated withmalignant glioma grade higher expression levels gradually increased, MVD ’shas gradually increased expression levels, and the expression of PDGFR-βand MVD showed a significant positive correlation (r=0.955, P=0.000<0.05). Scatter further evidence by their relevance, and between the twogroups were significant differences (P are equal to0.000). And a significantdifference between gender and age groups, and has clearly statisticallysignificant (Gender: Mann-Whitney U statistic is468.000P=0.004<0.05,Age: Mann-Whitney U statistic is354.500P=0.000<0.05).2RT-PCR results showed that: the difference between the degree ofPDGFR-β expression in normal brain tissue and various levels of humanglioma tissue with statistically significant (χ2=24.774P=0.000<0.05), withtumor increased malignancy, PDGFR-β expression was gradually increased.And between the two groups were significantly different (normal andMann-Whitney U statistic between low levels of0.000Z=-3.254P=0.000<0.05, Mann-Whitney U statistic between normal and high-level to0.000Z=-3.464P=0.000<Mann-Whitney U statistic0.05between low-level andhigh-level of0.000Z=-4.099P=0.000<0.05).Conclusions:1PDGFR-β expression in the human brain glioblastoma between thegroup and the control group with normal brain tissue statistically significantdifferences in normal brain tissue in the control group was significantly lowerthan the human brain glioblastoma group. Tip PDGFR-β expression in thepresence of the human brain and glioblastoma. PDGFR-β grade gliomas withelevated and elevated prompt, and the degree of malignancy of brainglioblastoma positive correlation.2MVD in human brain glioblastoma expression between the group andthe control group with normal brain tissue obvious differences, the fewestnumber in normal brain tissue, along with human glioma malignancy gradeincreased, MVD ’s expression level is gradually increased, and the degree of malignancy of brain glioblastoma positive correlation.3PDGFR-β expression and MVD in normal brain tissue and glioma has asignificant correlation with the PDGFR-β expression levels increased, MVDexpression level gradually increased.4and the difference between sex and age group, with a significantlystatistically significant, the incidence of gliomas can be drawn more men thanwomen, more than45years of age more than45years of age.5PDGFR-β expression may also further promote the adjustment ofglioma own environment, but also more conducive to the formation of gliomamicrovascular continue intensive, between the two are complementaryinteraction relationship, blocking PDGFR-β pathways of signal transductionpathway may block tumor cell growth and metastasis, blocking the generationand propagation of tumor microvessels, and may provide a new way ofthinking and direction for the treatment of glioma and gliomas could begenetic the new target for the treatment of.