The Study Of PERK-ATF4-CHOP Pathway In The Apoptosis Of SKOV3and VK3Resistance In SKOV3/DDP

Endoplasmic reticulum stress may play an important role inchemotherapy resistance of cancer. There are many signal pathwaysthat associated with cell’s survival and death. Our previousstudies found that PERK-KEAP1-Nrf2pathway is responsible forcisplatin resistance of ovarian cancer cells. Therefore, another pathway---PERK-ATF4-CHOP pathway is researched in order to reveal deepmechanisms in the apoptosis of SKOV3and VK3resistance inSKOV3/DDP.In order to study the funtion of PERK-ATF4-CHOP pathway in theapoptosis of SKOV3and VK3resistance in SKOV3/DDP, a reportedoxidants---vitamin K3,was used to induce oxidative stress in SKOV3cells and SKOV3/DDP cells in this study. We tested the apoptosis in thesetwo cell lines by MTT assay and flow cytometry. And we observed thechanges of proteins in PERK-ATF4-CHOP signal pathway by westernblot. The results showed that SKOV3/DDP cells had a strong ability toresistant the pro-apoptotic effect of VK3when treated with VK3.However, the apoptosis induced by PERK-ATF4-CHOP signalpathway was observed in SKOV3cells.In conclusion, our results showed that SKOV3cells got apoptisis induced by ERS on the effect of VK3, however, SKOV3/DDP cells canresist ERS induced by VK3,and PERK-ATF4-CHOP pathwaywas not actived in SKOV3/DDP cells. Therefore, we suppose thatthe PERK-ATF4-CHOP pathway play an important role in drugresistance in the apoptosis of SKOV3and VK3resistance inSKOV3/DDP, and these results might provide new perspectiveto overcome drug resistance in ovarian cancer.