Effect Of MiR-145on Prostate Cancer PC-3Cell Proliferation And Related Genes’ Expression

Objective: To investigate the effects of miR-145on cell proliferation, cell apoptosisand the expression of related genes on prostate cancer PC-3cell line.Methods: Prostate cancer cell lines PC-3was routinely cultured in vitro. PC-3cellswere randomly allocated into3groups: blank control group without any vector transfection,negative control group, which only transfected with the vector without the target gene, andmiR-145group that transfected with lentiviral vectors carrying miR-145.72hours afterinfection, the cell infection efficiency of were determined by scanning the greenfluorescent protein with fluorescence microcopy. Expression level of miR-145wasdetected by fluorescence quantitative polymerase chain reaction(qPCR). Cell proliferationwas examined by MTT assay. Cell apoptosis was detected with flow cytometry. Thepreviously predicted target genes(（C-MYC， IRS1，IGF1R，CDKN1A，CCNA2） ofmiR-145was examined by reverse transcription polymerase chain reaction (RT-PCR).Results:72hours after the transfection,the transfection efficiency that we observedwas over80%. qPCR results showed that miR-145expression in the miR-145group wassignificantly increased as compared with negative control group and blank group,MTTassay results showed that the cell proliferation after miR-145overexpression aresignificantly reduced than that in negative control group and blank group. Flow cytometryalso detected that the cell apoptosis increased after miR-145overexpressed. and IRS1genereduced after miR-145expressed in PC-3cells. All these differences were statisticallysignificant（P<0.05）.Conclusion: miR-145could inhibit cell growth and induce cell apoptosis in PC-3cells. MiR-145overexpression can reduce IRS1gene expression.