Research Of Methods For Preparing Rabbit Carotid Artery’Sacellular Matrix

Background:Cardiovascular diseases seriously damage human health, vascular reconstruction acts as a very important role in clinical field, so finding suitable vascular grafts has become an important research project which causes for concern. Currently, there are some biological materials for vascular scaffold clinically, including natural blood vessels, acellular matrix and artificial substitutes. Tissue engineering and regenerative medicine develope rapidly, and biological xenotransplantation of blood vessels has become an important research field currently. The developed materials have achieved many good results as large and medium caliber of vascular substitutes. However, the substitutes for small-diameter blood vessels (diameter<6mm) have low patency, the artificial vascular graft witch can replace small arteries has not obtained satisfactory results. It’s just because the implanted vascular’s intima becomes thick easily and forms thrombus easily. It’s urgent to find suitable small-diameter vascular substitutes clinically.In this study, we use a variety of methods to remove cells on rabbit carotid artery to prepare acellular vascular scaffold. Then we evaluate the acellular scaffolds prepared by different methods. Our purpose is to explore a more appropriate method for the preparation of acellular vascular scaffold of rabbit carotid artery.Method:We combined physical treatment, chemical treatment and enzymatic treatment to remove cells to prepare the acellular vascular scaffold of rabbit carotid artery. Tissue staining、electron microscopy%evaluation of cell toxicity and subcutaneous implantation were performed to observe the construction of the acellular vascular scaffold, cytotoxicity and immunogenicity, respectively.Results:We detected three preparation methods for preparation of decellularized rabbit carotid artery. They can remove cell components completely, there are no residual cells and cell fragments; extracellular matrix and three-dimensional structure of vascular scaffold are well preserved; the mechanical properties of the scaffolds before and after decellularization were not significantly changed and decellularized scaffolds has low cytotoxicity and immunogenicity.Conclusion:To prepare decellular vascular scaffold, we explored several methods to remove cells on rabbit carotid artery. The methods are able to completely remove the cell components of rabbit carotid artery vessel walls and preserve extracellular matrix components. The several methods can serve references for preparing tissue engineered blood vessel with three-dimensional structure.