Expression And Clinical Significalicance Of TGF-β1and CD31in Papillary Thyroid Carcinoma

Objective:By testing the expression of transforming growth factor-β1(TGFβ1),CD31and assessing microvessel density(MVD) marked by CD31in papillary thyroid carcinoma(PTC), we tried to discuss their relationship with PTC.Method:1We investigated the expression of TGF-β1,CD31and assessed MVD in60PTCs,20adjacent normal thyroid tissues by immunohistochemistry. And compared these former factors with clinical pathological datas of PTC.2Western blot was used to detect the expression of TGF-β1,CD31in20PTCs, and10adjacent normal tissues of PTC.Result:1Expression of TGFβ1protein: TGF-(31was mainly expressed in the cytoplasm of tumor cells,partly in caryon,distributed mainly in marginal zone of the tumor and a small quantity of normal tissues. Statistical analysis revealed that TGF-β1-positive stained rate was significant higher in PTCs (70.0%) than that in normal thyroid tissues (20.0%)(P<0.01). There were significant difference between PTC group and normal thyroid tissue group (P<0.01).2Expression of CD31protein: CD31protein was mainly expressed in the the interstitial vascular endothelial cell of the thyroid carcinoma marginal zone. Statistical analysis revealed that MVD was112.11±6.13in PTCs, and that was44.83±6.22in normal thyroid tissues (P<0.01). There were significant difference between PTC group and normal thyroid tissue group (P<0.01).3The relationship of TGF-β1with clinical pathological datas of PTC: Statistical analysis revealed that the expression of TGF-β1protein in PTCs had no significant relation with sex, age, tumor size (P>0.05), but it was correlated with lymph node metastasize, UICC stage (P<0.05). Expression rate of TGFβ1protein with lymph node metastasize (78.1%) was significant higher than that in without (57.1%)(P<0.05). Accompany with the step up of UICC stage, the expression rates of TGF-β1protein were rising (Ⅰ42.9%, Ⅱ58.3%,11181.3%, IV88.9%)(P<0.05). TGF-β1-positive stained rate was significant higher in advanced stage (Ⅲ, Ⅳ)(85.3%) than that in early stage (Ⅰ, Ⅱ)(50.0%)(P<0.01).4The relationship of CD31with clinical pathological datas of PTC: Statistical analysis revealed that the expression of CD31protein in PTC had no significant relation with sex, age, tumor size (P>0.05), but it was correlated with lymph node metastasize, UICC stage (P<0.05). Expression rate of CD31protein with lymph node metastasize (84.0%) was significant higher than that in without (55.6%)(P<0.05). Accompany with the step up of UICC stage, the expression rates of CD31protein were rising (Ⅰ35.7%,Ⅱ66.7%, Ⅲ75.0%, Ⅳ100.0%)(P<0.01). There were significant differences when the IV group compared with the others (P<0.01). CD31-positive stained rate was significant higher in advanced stage (Ⅲ, Ⅳ)(88.2%) than that in early stage (Ⅰ, Ⅱ)(50.0%)(P<0.01).5The relationship of MVD with the expression of TGF-β1、CD31in PTC: The mean MVD value in group with TGF-β1positive expressions was significantly higher than that in the negative group; The mean MVD value in group with CD31positive expressions was significantly higher than that in the negative group; the MVD increased with the increased expression of TGF-β1CD31.6The outcome of Western blot: The value of OD of TGF-β1-positive expression in PTC was significant increased (2.487±0.037) than that of thyroid normal tissue (0.184±0.018)(P<0.01). There were significant differences when the groups compared with each other (P<0.01).The result was concordant with immunohistochemistry.The value of OD of CD31-positive expression in PTC was significant increased (2.464±0.032) than that of thyroid normal tissue(0.174±0.013)(P<0.01),too. There were significant differences when the groups compared with each other (P<0.01).The result was concordant with immunohistochemistry.Conclusion:1Compared with thyroid normal tissue, increased expression of TGFβ1, CD31protein and MVD in PTC indicated that TGFβ1and CD31were closely correlated with carcinogenesis and metastasis of PTC.2The expression rates of TGF-β1and CD31protein were correlated with lymph node metastasize and UICC stage which manifest that both of them may have some effects in tumor malignancy and invasion. This may become an outcome indicator for PTC.3The close relationship of TGF-β1protein and MVD in PTC indicated that TGF-β1may mediated angiogenesis,and by this it may have provided an essensial enviroment for tumor cell growth, malignancy and invasion.