Protective Effect Of GIK On Endotoxemia Liver Injury In Rats

Objective: Through establishment of endotoxemia liver injury of ratmodel which give GIK treatment to study whether GIK would have protectiveeffect for liver injury caused by endotoxin.Method:60SD rats were randomly divided into three groups: Normalcontrol group (NS, n=20), rats received an intraperitoneal injection of normalsaline8ml/kg for three days,2hours after the first injection, intravenousinfusion normal saline4ml/kg/h, lipopolysaccharide group (LPS, n=20): ratsreceived an intraperitoneal injection of LPS8mg/kg for three days,2hoursafter the first injection, intravenous infusion of normal saline4ml/kg/h. GIKgroup (GIK, n=20): rats received an intraperitoneal injection of LPS8mg/kgfor three days,2hours after the first injection,intravenous infusion of GIK4ml/kg/h (G:200g/l, insulin:60IU/l, KCL:60mmol/l). According to the liquidtreatment time after LPS injection, We divided it into3days group and5daysgroup, each secondary group had10rats. To observe and compare thebehavioristic change of rats in each group, detected the serum aspartateaminotransferase (AST) and alanine aminotransferase(ALT) by biochemicalanalyzer. Took the liver tissue to make into homogenate and paraffin section,The content of tumor necrosis factor alpha, interleukin10, and nuclearfactor-kappa B in liver homogenate were detected by ELISA. Observed thechanges of hepatic pathology by microscope and evaluated the injury degreeof liver. At last, the results were statistically analyzed.Results:1. The rats in LPS group show that activity reduced, fatigue,poor appetite, pilo-erection, shortness of breath, cyanosis, weight loss. Rats inGIK group were better than that of the in LPS group.2. ALT and AST levels: Blood ALT and AST have the same change trend,3days after LPS injection,the level of AST and ALT from the LPS group and GIK group weresignificantly increased when compared with the normal control group(p<0.05), but there was no statistical significance between the LPS group andGIK group. While5days after LPS injection, compared with the normalgroup, the level of ALT and AST in LPS group were significantly increased(p<0.05), and the GIK group had no significant difference (p>0.05). Whencompared with the LPS group, the GIK group was significantly decreased(p<0.05).3. Pathological changes in liver tissues: the liver cells were integrity,no degeneration and necrosis in the normal control group,3days after LPSinjection, the liver cells of LPS group appeared swelling, cytoplasmicvacuoles, and inflammatory cell infiltration. The GIK group was better thanthe LPS group. While5days after LPS injection, the liver of LPS groupappeared hemorrhage and necrosis, and the liver of GIK group were integrity,no degeneration and necrosis but inflammatory cell infiltration, comparedwith normal control group, the score level of liver injury of the other twogroups were significant differences in statistically, LPS group have heavierdamage than GIK group (p<0.05).4. The levels of TNF-a and IL-10:3daysafter LPS injection, the level of TNF-a from the LPS group and GIK groupwere significantly increased when compared with the normal control group(p<0.05), compared with the LPS group, the GIK group was significantlydecreased (p<0.05). While5days after LPS injection, compared with thenormal group, the level of TNF-a in LPS group and GIK group weresignificantly increased (p<0.05). When compared with the LPS group, theGIK group was significantly decreased (p<0.05). The value of IL-10in LPS5days group and GIK group were significantly higher than that in the normal control group (p<0.05), in which GIK5days group was the highest, the levelof IL-10in each secondary GIK group were higher than those in LPS group,there was statistically significant difference between the group of5days(P<0.05).5. Liver homogenate NF-кB level:3days after LPS injection,the level of NF-кB from the LPS group and GIK group were significantlyincreased when compared with the normal control group(p<0.05), but therewas no statistical significance between the LPS group and GIK group. While5days after LPS injection, compared with the normal group, the level ofNF-кB in LPS group and GIK group were significantly increased (p<0.05).When compared with the LPS group,the GIK group was significantlydecreased (p<0.05).Conclusion: Intraperitoneal injection of endotoxin can cause liver injuryin rats, resulting in the change of liver function and liver cell damage. GIKhas protective effects on LPS induced liver injury in rats.