The Relevant Research On The Blood Stasis Svndrome Of Premature Coronary Heart Disease And ACE Gene、MEF2A Gene Single Nucleotide Polymorphism

Objective:To investigate the relationship of the the blood stasis syndrome of premature coronary heart disease(PCHD) with Angiotensin Converting Enzyme(ACE)gene single nucleotide polymorphism、Myocyte Enhancer Factor-2a (MEF2A) gene single nucleotide polymorphism.Method:Choose three SNPs rs4343、rs4293and rs4267385in the ACE gene, and two SNPs rs2033547、rs34851361in the MEF2A gene,detecting the SNPs of21cases in the group of blood-stasis syndrome of PCHD,30cases in the group of non-blood-stasis syndrome of PCHD,25cases in the group of blood-stasis syndrome of non-PCHD and20cases in the control group base on the technology of the time of flight mass spectrometry technique(TOF MS), compare the allele and genotype frequencies of each group.Result:①There are two alleles G. A and three genetype GGGA.AA in rs4343, rs4293,two alleles C.T and three genetype CC.CT.TT in rs4267385and two alleles A.T and three genetype AA.AT. TT in rs2033547、rs34851361.②In the four groups, the polymorphism of rs4293and rs4267385in ACE gene and rs2033547、rs34851361in MEF2A gene has no statistical significance(P<0.05).③The difference of the genotype and allele frequency in rs4343in four groups has statistical significance(P<0.05),compare with control group,the difference of allele frequency in rs4343in blood-stasisi of PCHD group and non-blood stasis of PCHD has statistical significance(P<0.05).④The G allele frequency in PCHD group and non-blood stasis of PCHD is higher than control group (P<0.05or P<0.01). Compare the OR value of each group who carries the G allele frequency, the person who carries G allele is3.6times than who not carries G allele with PCHD(P<0.05).Conclusion:①There are two alleles G.A and three genetype GG. GA.AA in rs4343, rs4293,two alleles C.T and three genetype CC.CT. TT in rs4267385and two alleles A.T and three genetype AA.AT.TT in rs2033547、rs34851361.②The ACE gene SNP namely rs4293and rs4267385maybe not associated with PCHD.The ACE gene SNP namely rs4343is associated with PCHD,and G allele is the risk factor of PCHD,but not be the risk factor of blood stasis;③The MEF2A gene SNP namely rs2033547、rs34851361maybe not associated with PCHD.